207738-08-7

Basic information

• Product Name: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%
• Synonyms: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%;3,3μ,5,5μ-Tetramethylbenzidine hydrate dihydrochloride;3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate,TMB dihydrochloride;TMB (hydrochloride hydrate);3,3',5,5'-tetraMethylbiphenyl-4,4'-diaMine dihydrochloride hydrate (1:2:);3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate;3,3',5,5'-TetraMethylbenzidine dihydrochloride hydrate 97%;3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate Vetec(TM) reagent grade, >=98%
• CAS NO: 207738-08-7
• Molecular Formula: C16H20N2.2ClH
• Molecular Weight: 313.27
• EINECS: 1312995-182-4
• Mol File: 207738-08-7.mol
• Article Data: 0

Chemical Properties

• Melting Point: >300°C
• Boiling Point: 459.8oC at 760 mmHg
• Flash Point: 231.9oC
• Appearance: /
• Storage Temp.: 2-8°C
• Solubility: Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03
• Sensitive: Moisture & Light Sensitive
• CAS DataBase Reference: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%(CAS DataBase Reference)
• NIST Chemistry Reference: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%(207738-08-7)
• EPA Substance Registry System: 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%(207738-08-7)

Safety Data

• Statements: 22
• Safety Statements: 22-24/25-25-24
• WGK Germany: 3
• TSCA: Yes
• Hazardous Substances Data: 207738-08-7(Hazardous Substances Data)

Usage

Description

TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

Chemical Properties

Off-white fluffy powder

Uses

Different sources of media describe the Uses of 207738-08-7 differently. You can refer to the following data:
1. 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.
2. Convenient water-soluble form of TMB for determination of peroxidase.
3. 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).

General Description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.