3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate

Base Information

• Chemical Name: 3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate
• CAS No.: 207738-08-7
• Molecular Formula: C16H20 N2 . 2 Cl H . x H2 O
• Molecular Weight: 331.28056
• Hs Code.: 29215900
• European Community (EC) Number: 688-511-0
• Mol file: 207738-08-7.mol

Synonyms:3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate;207738-08-7;3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate;AKOS022184577;AS-10088;PD020837;T-2155;3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine, dihydrochloride, dihydrate;4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline;dihydrate;dihydrochloride

Chemical Property of 3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate

Chemical Property:

• Melting Point: >300oC
• Boiling Point: 459.8oC at 760 mmHg
• Flash Point: 231.9oC
• PSA: 52.04000
• LogP: 6.51800
• Storage Temp.: 2-8°C
• Sensitive.: Moisture & Light Sensitive
• Solubility.: Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03
• Hydrogen Bond Donor Count: 6
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 1
• Exact Mass: 348.1371335
• Heavy Atom Count: 22
• Complexity: 226

Purity/Quality:

99% ^*data from raw suppliers

3,3'',5,5''-TetramethylbenzidineDihydrochlorideHydrate(1:2:x) ^*data from reagent suppliers

Safty Information:

• Statements: 22
• Safety Statements: 22-24/25-25-24

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C.O.O.Cl.Cl
• Description: TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.
• Uses: 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine. Convenient water-soluble form of TMB for determination of peroxidase. 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).