53-73-6 Usage
Description
ASN-ARG-VAL-TYR-VAL-HIS-PRO-PHE, also known as Angiotensin amide (Hypertensin), is a synthetic polypeptide with the sequence 1-L-aspariginyl-5-L-valine angiotensinoctapeptide. It possesses twice the pressor activity of angiotensin II and is used in the pharmaceutical industry for its potent effects on blood pressure regulation.
Uses
Used in Pharmaceutical Industry:
ASN-ARG-VAL-TYR-VAL-HIS-PRO-PHE is used as a pressor agent for the treatment of various hypotensive states. It is particularly useful in controlling acute hypotension during the administration of general anesthetics that sensitize the heart to the effects of catecholamines. The pressor effect of this polypeptide is due to an increase in peripheral resistance, as it constricts resistance vessels with little or no stimulating action on the heart and minimal effect on the capacitance vessels.
Additionally, it is available as a lyophilized powder for injection, with dosages ranging from 0.5–2.5 mg diluted in 500 mL of sodium chloride injection or 5% dextrose for injection, to be administered by continuous infusion.
Originator
Hypertensin,Ciba,W. Germany,1961
Manufacturing Process
48 mg (0.042 mmol) of L-asparaginyl-L-arginyl-L-valyl-L-tyrosyl-L-valyl-Lhistidyl-
L-prolyl-L-phenylalanine methyl ester trihydrochloride are suspended
in 0.5 ml of methanol, and treated gradually in the course of one hour with
0.3 ml of N-caustic soda solution (about 7 equivalents) so that the pH value of
the solution is maintained between 10.5 and 11.5. After a further 30 minutes
the solution is freed from methanol under vacuum at room temperature,
adjusted with 1 N acetic acid to pH 7.4 and lyophilized. The residual mixture
of free peptide and inorganic salts (79 mg) is fractionated by countercurrent
distribution in the system butanol/0.1 N ammonium hydroxide. The pure
octapeptide is obtained as a colorless powder which is soluble in water and
methanol, more sparingly soluble in ethanol, and insoluble in acetone.
Check Digit Verification of cas no
The CAS Registry Mumber 53-73-6 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 5 and 3 respectively; the second part has 2 digits, 7 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 53-73:
(4*5)+(3*3)+(2*7)+(1*3)=46
46 % 10 = 6
So 53-73-6 is a valid CAS Registry Number.
InChI:InChI=1/C49H70N14O11/c1-26(2)39(61-42(67)33(12-8-18-55-49(52)53)57-41(66)32(50)23-38(51)65)45(70)58-34(20-29-14-16-31(64)17-15-29)43(68)62-40(27(3)4)46(71)59-35(22-30-24-54-25-56-30)47(72)63-19-9-13-37(63)44(69)60-36(48(73)74)21-28-10-6-5-7-11-28/h5-7,10-11,14-17,24-27,32-37,39-40,64H,8-9,12-13,18-23,50H2,1-4H3,(H2,51,65)(H,54,56)(H,57,66)(H,58,70)(H,59,71)(H,60,69)(H,61,67)(H,62,68)(H,73,74)(H4,52,53,55)/t32-,33-,34-,35-,36-,37-,39-,40-/m0/s1
53-73-6Relevant articles and documents
Interfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry: Label-free content analysis of single droplets
Kuester, Simon K.,Fagerer, Stephan R.,Verboket, Pascal E.,Eyer, Klaus,Jefimovs, Konstantins,Zenobi, Renato,Dittrich, Petra S.
, p. 1285 - 1289 (2013)
Droplet-based microfluidic systems have become a very powerful tool to miniaturize chemical and biological reactions. However, droplet content analysis remains challenging and relies almost exclusively on optical methods such as fluorescence spectroscopy. Hence, labeling of the analyte is typically required which impedes a more universal applicability of microdroplets. Here we present a novel interface coupling droplet microfluidics and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for label-free content analysis of single droplets. Nanoliter aqueous droplets immersed in perfluorinated oil are created in a microfluidic T-junction, transferred into a capillary, and deposited on a high-density microarray MALDI plate mounted on a motorized xy-stage. The fully automated system is robust and reliable due to two unique features. First, a simple optical droplet detection system is used to synchronize stage movement and exit of droplets from the capillary. Second, the microarray plate contains an array of over 26 000 hydrophilic spots within a hydrophobic coating, each spot acting as a recipient to confine the droplets and to prevent cross-contamination. The MALDI matrix can also be applied using our system by spotting matrix droplets on the microarray in a separate run. To demonstrate the potential of our system, we studied the enzymatic cleavage of angiotensin I by angiotensin converting enzyme and monitored the increasing concentration of the product angiotensin II over time. The interface provides a robust and fully automated method for rapid label-free and information-rich content analysis of single droplets. With the high number of droplets per plate, this method is particularly suitable for high-throughput screening applications.
INHIBITORY OCTAPEPTIDES ANGIOTENSIN II
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, (2008/06/13)
Octapeptide derivatives characterized by an oxygen or sulfur-containing moiety in the C-terminal position are potent inhibitors of the pharmacological effects of Angiotensin and possess the additional advantage of a favorable antagonist/agonist ratio.
