442632-72-6

Basic information

• Product Name: CK-636
• Synonyms: CK-636;N-[2-(2-Methyl-1H-indol-3-yl)ethyl]-2-thiophenecarboxamide;CK-0944636;CK-636 N-[2-(2-Methyl-1H-indol-3-yl)ethyl]-2-thiophenecarboxamide;N-[2-(2-Methyl-1H-indol-3-yl)ethyl]-2-thiophenecarboxamide CK636;CK-636, >=98%
• CAS NO: 442632-72-6
• Molecular Formula: C16H16N2OS
• Molecular Weight: 284.37604
• EINECS: 200-256-5
• Product Categories: Inhibitors
• Mol File: 442632-72-6.mol
• Article Data: 0

Chemical Properties

• Appearance: peach to light tan/
• Storage Temp.: 2-8°C
• Solubility: DMSO: ≥20mg/mL
• CAS DataBase Reference: CK-636(CAS DataBase Reference)
• NIST Chemistry Reference: CK-636(442632-72-6)
• EPA Substance Registry System: CK-636(442632-72-6)

Safety Data

• Hazard Codes: Xn
• Statements: 22
• WGK Germany: 3
• Hazardous Substances Data: 442632-72-6(Hazardous Substances Data)

Usage

Description

CK-636 is an Arp2/3 complex inhibitor with varying IC50 values for inhibiting actin polymerization induced by different sources of Arp2/3 complex, specifically 4 μM for human, 24 μM for fission yeast, and 32 μM for bovine Arp2/3 complex. It is a small molecule that interacts with the Arp2/3 complex to prevent actin polymerization, which plays a crucial role in regulating the actin cytoskeleton.

Uses

Used in Cellular Biology Research:
CK-636 is used as a research tool for studying the role of the Arp2/3 complex in cellular processes. Its ability to inhibit actin polymerization allows researchers to investigate the effects of disrupting the actin cytoskeleton on various cellular functions and mechanisms.
Used in Drug Development:
CK-636 is used as a lead compound in the development of new drugs targeting the Arp2/3 complex. Its inhibitory properties make it a potential candidate for treating diseases and conditions associated with abnormal actin cytoskeleton regulation, such as certain types of cancer and immune disorders.
Used in Cancer Therapy:
CK-636 is used as a potential therapeutic agent for cancer treatment. By inhibiting the Arp2/3 complex and disrupting the actin cytoskeleton, it may help to prevent tumor cell migration, invasion, and metastasis, thus offering a novel approach to cancer therapy.
Used in Immunology Research:
CK-636 is used as an immunological research tool to study the role of the Arp2/3 complex in immune cell function. Its inhibitory effects on actin polymerization can provide insights into the mechanisms underlying immune cell migration, activation, and response to pathogens.

In vitro

CK-636 inhibits actin polymerization mediated by Arp2/3 complex in live cells, and reduces the formation of actin filament comet tails by Listeria in infected SKOV3 cells. CK-636 inhibits lamellipodia formation at the leading edges of migrating T cells, and causes reduced velocity. In addition, a substantial fraction of CK-636-treated T cells on zigzag patterns with an acute turning angle are trapped near the interfaces formed by the turning points of zigzag patterns.