Potentiometric detection of N,N′-Diethylaminoethanol (cas 100-37-8) and lysosomotropic amino alcohols in cation exchange high-performance liquid chromatography systems

Add time:07/24/2019    Source:sciencedirect.com

Potentiometric detection of N,N′-diethylaminoethanol (DEAE) and a series of physiologically relevant lysosomotropic amino alcohols was demonstrated in cation exchange HPLC. Poly (vinyl chloride) (PVC) based liquid membrane electrode coatings were used which contained the lipophilic cation exchanger tetrakis(p-chlorophenyl)borate (TCPB) either alone or in combination with one of the macrocyclic ionophores hexakis-(2,3,6-tri-O-octyl)-α-cyclodextrin (CDX), dibenzo-18-crown-6 (CRW) or calix[6]arene hexaethylacetate ester (CLX). For all PVC membrane electrodes used, improved sensitivity over UV detection at 210 nm was obtained for the analyzed aliphatic amino alcohols. Especially, addition of CLX to the PVC-based membrane leads to highly enhanced detection limits for the low molecular weight lysosomotropic amino compounds, i.e. aminoethanol and methylamine. The performance of the proposed potentiometric detection method was demonstrated in cation exchange HPLC modes employing two different aqueous mobile phase systems with pH∗ 2.30 comprising phosphoric or acetic acid with acetonitrile as organic modifier. Detection limits of 1.1×10−8 and 2.4×10−8 M (injected concentrations) were achieved, respectively, for N-methylaminoethanol and hexylamine by using a CLX based electrode. Sensitive and reliable determination of DEAE, a local anaesthetic agent, after liquid–liquid or SPE extraction from fortified bovine serum samples was obtained with the HPLC method coupled with the proposed potentiometric detection system. A linear free-energy relationship (LFER) based on a general solvation equation was used to predict detection limits of amino alcohols from their molecular structure in the developed potentiometric detectors employing CLX and CRW.

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