979-88-4

Basic information

• Product Name: 2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt
• Synonyms: 2,2'-BICINCHONINIC ACID DISODIUM SALT;2,2'-BIQUINOLINE-4,4'-DICARBOXYLIC ACID DISODIUM SALT;2,2'-BIQUINOLINE-4,4'-DICARBOXYLIC ACID DISODIUM SALT TRIHYDRATE;4,4'-DICARBOXY-2,2'-BIQUINOLINE DISODIUM SALT;4,4'-DICARBOXY-2,2'-BIQUINOLINE, SODIUM SALT;BCA DISODIUM SALT;BCA;BICINCHONINIC ACID DISODIUM SALT
• CAS NO: 979-88-4
• Molecular Formula: C20H11N2Na2O4
• Molecular Weight: 389.29
• EINECS: 629-761-2
• Product Categories: marker
• Mol File: 979-88-4.mol
• Article Data: 0

Chemical Properties

• Melting Point: 300℃
• Boiling Point: 626.2 °C at 760 mmHg
• Flash Point: 332.5 °C
• Appearance: white to light yellow/Powder/Chunks
• Storage Temp.: 2-8°C
• Solubility: 0.5% sodium bicarbonate: soluble50mg/mL
• Water Solubility: Soluble in water.
• Stability: Hygrscopic
• CAS DataBase Reference: 2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: 2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt(979-88-4)
• EPA Substance Registry System: 2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt(979-88-4)

Safety Data

• Hazard Codes: Xn,Xi
• Statements: 36/37-42-36/38-36/37/38-20/21/22
• Safety Statements: 22-26-36/37-45-36
• WGK Germany: 3
• TSCA: Yes
• Hazardous Substances Data: 979-88-4(Hazardous Substances Data)

Usage

Description

2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt, also known as BCA, is a colorimetric reagent used for the detection and quantification of copper (Cu+) and protein. It forms a stable 2:1 complex with Cu+, which has an absorbance maximum at 562 nm, making it a reliable indicator for these assays.

Uses

Used in Analytical Chemistry:
2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt is used as a reagent for the detection of copper (Cu+), forming a stable complex that aids in accurate measurement.
Used in Protein Quantitation Assays:
In the colorimetric BCA protein quantitation assay, 2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt is used to convert Cu2+ to Cu+ as a function of protein concentration under alkaline conditions, enabling the determination of protein levels.
Used in Reducing Sugar Detection:
2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt hydrate is used to monitor the levels of reducing sugars, which helps in the detection of chitinases and the determination of cellulose reducing ends (micromoles/g cellulose).
Used in Copper Determination:
2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt is useful for the determination of copper, providing a reliable method for measuring copper concentrations in various samples.
Used in Various Industries:
2,2'-Biquinoline-4,4-dicarboxylic acid disodium salt is used across different industries for its applications in analytical chemistry, protein quantitation, reducing sugar detection, and copper determination, making it a versatile reagent for research and quality control purposes.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.