862778-54-9

Basic information

• Product Name: N,N-Bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-L-glutaMic Acid 1-(1,1-DiMethylethyl) 5-(PhenylMethyl) Ester
• Synonyms: N,N-Bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-L-glutaMic Acid 1-(1,1-DiMethylethyl) 5-(PhenylMethyl) Ester
• CAS NO: 862778-54-9
• Molecular Formula: C28H43NO8
• Molecular Weight: 521.64292
• Mol File: 862778-54-9.mol
• Article Data: 12

Chemical Properties

• Appearance: /
• Solubility: Chloroform, DCM, DMSO
• CAS DataBase Reference: N,N-Bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-L-glutaMic Acid 1-(1,1-DiMethylethyl) 5-(PhenylMethyl) Ester(CAS DataBase Reference)
• NIST Chemistry Reference: N,N-Bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-L-glutaMic Acid 1-(1,1-DiMethylethyl) 5-(PhenylMethyl) Ester(862778-54-9)
• EPA Substance Registry System: N,N-Bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-L-glutaMic Acid 1-(1,1-DiMethylethyl) 5-(PhenylMethyl) Ester(862778-54-9)

Safety Data

• Hazardous Substances Data: 862778-54-9(Hazardous Substances Data)

Usage

Description

N,N-Bis[2-(1,1-dimethylethoxy)-2-oxoethyl]-L-glutamic Acid 1-(1,1-Dimethylethyl) 5-(Phenylmethyl) Ester is a protected intermediate in the synthesis of chelating agents. It plays a crucial role in the development of fluorescence labeling techniques for histidine-tagged proteins to lipid membranes. N,N-Bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-L-glutaMic Acid 1-(1,1-DiMethylethyl) 5-(PhenylMethyl) Ester is instrumental in advancing the study and monitoring of protein interactions within cellular environments.

Uses

Used in Biomedical Research:
N,N-Bis[2-(1,1-dimethylethoxy)-2-oxoethyl]-L-glutamic Acid 1-(1,1-Dimethylethyl) 5-(Phenylmethyl) Ester is used as a protected intermediate for the synthesis of chelating agents in biomedical research. It aids in the fluorescence labeling of histidine-tagged proteins to lipid membranes, which is essential for studying and monitoring protein interactions in cells. This application is particularly valuable for gaining insights into cellular processes and mechanisms, contributing to the development of new therapeutic strategies and diagnostic tools.

Upstream product

• 5292-43-3 bromoacetic acid tert-butyl ester 
• 80165-23-7 (S)-2-aminopentanedioic acid 5-benzyl ester 1-tert-butyl ester 
• 105590-97-4 L-glutamic acid γ-benzyl α-tert-butyl ester hydrochloride 
• 25456-75-1 t-butoxycarbonyl-γ-aminobutyric acid 

Downstream Products

• 862778-55-0 2-(bis-tert-butoxycarbonylmethylamino)-pentanedioic acid 1-tert-butyl ester 
• 862778-55-0 Glu-NTA 

Relevant articles and documents

Development of an ON/OFF switchable fluorescent probe targeting His tag fused proteins in living cells

The fluorescent labeling of target proteins is useful for analyzing their functions and localization in cells, and several fluorescent probes have been developed. However, the fusion of tags such as green fluorescent protein (GFP) to target proteins occas

Development of a Small Hybrid Molecule That Mediates Degradation of His-Tag Fused Proteins

In recent years, the induction of target-protein degradation via the ubiquitin-proteasome system (UPS) mediated by small molecules has attracted attention, and this approach has applications in pharmaceutical development. However, this technique requires a ligand for the target protein that can be incorporated into tailor-made molecules, and there are many proteins for which such ligands have not been found. In this study, we developed a protein-knockdown method that recognizes a His-tag fused to a protein of interest. This strategy theoretically allows comprehensive targeting of proteins of interest by a particular molecule recognizing the tag. As expected, our hybrid molecule 10 [SNIPER(CH6)] efficiently degraded His-tagged CRABP-II and Smad2 in cells. This system provides an easy method to determine the susceptibility of proteins of interest to UPS-mediated degradation. Furthermore, we hope that this method will become an efficient tool to analyze the function of the UPS.

High-Affinity Copolymers Inhibit Digestive Enzymes by Surface Recognition

This account presents a general method for the construction of polymeric surface binders for digestion enzymes. Two prominent parts, namely, the modification of the copolymer composition and the screening assay for the most powerful inhibitors are both am