85138-49-4 Usage
Description
BCECF, also known as 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, is a fluorescent probe used for measuring intracellular pH and monitoring pH changes in various biological systems.
Used in Biomedical Research:
BCECF is used as a pH indicator for measuring intracellular pH in epithelial cell monolayers, interstitial spaces of normal and neoplastic tissue, and isolated cell fractions. It provides a reliable measure of in vitro cellular cytotoxicity.
Used in Fluorescence-based Screening Assays:
BCECF is used as a pH-sensitive probe in fluorescence-based screening assays to determine the pH in various biological samples. Its fluorescence properties allow for accurate and sensitive pH measurements, making it a valuable tool in research and drug discovery.
Biochem/physiol Actions
Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.
Check Digit Verification of cas no
The CAS Registry Mumber 85138-49-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,5,1,3 and 8 respectively; the second part has 2 digits, 4 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 85138-49:
(7*8)+(6*5)+(5*1)+(4*3)+(3*8)+(2*4)+(1*9)=144
144 % 10 = 4
So 85138-49-4 is a valid CAS Registry Number.
InChI:InChI=1/C27H20O11/c28-19-10-21-17(7-12(19)2-5-23(30)31)25(16-9-14(26(34)35)1-4-15(16)27(36)37)18-8-13(3-6-24(32)33)20(29)11-22(18)38-21/h1,4,7-11,28H,2-3,5-6H2,(H,30,31)(H,32,33)(H,34,35)(H,36,37)