621-91-0Relevant articles and documents
Synthesis of functionalized copillar[4+1]arenes and rotaxane as heteromultivalent scaffolds
Chen, Wenzhang,Mohy Ei Dine, Tharwat,Vincent, Stéphane P.
supporting information, p. 492 - 495 (2021/01/25)
In this study, novel copillar[4+1]arenes were used as central heteromultivalent scaffolds via orthogonal couplings with a series of biologically relevant molecules such as carbohydrates, α-amino acids, biotin and phenylboronic acid. Further modifications by introducing maleimides or cyclooctyne groups provided molecular probes adapted to copper-free click chemistry. An octa-azidated fluorescent rotaxane bearing two distinct ligands was also generated in a fully controlled manner.
A Synthetic Fluorescent Mitochondria-Targeted Sensor for Ratiometric Imaging of Calcium in Live Cells
Pendin, Diana,Norante, Rosa,De Nadai, Andrea,Gherardi, Gaia,Vajente, Nicola,Basso, Emy,Kaludercic, Nina,Mammucari, Cristina,Paradisi, Cristina,Pozzan, Tullio,Mattarei, Andrea
supporting information, p. 9917 - 9922 (2019/06/24)
Ca2+ handling by mitochondria is crucial for cell life and the direct measure of mitochondrial Ca2+ concentration in living cells is of pivotal interest. Genetically-encoded indicators greatly facilitated this task, however they require demanding delivery procedures. On the other hand, existing mitochondria-targeted synthetic Ca2+ indicators are plagued by several drawbacks, for example, non-specific localization, leakage, toxicity. Here we report the synthesis and characterization of a new fluorescent Ca2+ sensor, named mt-fura-2, obtained by coupling two triphenylphosphonium cations to the molecular backbone of the ratiometric Ca2+ indicator fura-2. Mt-fura-2 binds Ca2+ with a dissociation constant of ≈1.5 μm in vitro. When loaded in different cell types as acetoxymethyl ester, the probe shows proper mitochondrial localization and accurately measures matrix [Ca2+] variations, proving its superiority over available dyes. We describe the synthesis, characterization and application of mt-fura-2 to cell types where the delivery of genetically-encoded indicators is troublesome.
Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention
Takei, Yoshiaki,Murata, Atsushi,Yamagishi, Kento,Arai, Satoshi,Nakamura, Hideki,Inoue, Takafumi,Takeoka, Shinji
supporting information, p. 7313 - 7315 (2013/09/23)
The powerful strategy of "intracellular click reaction" was used to retain a chemical Ca2+ indicator in the cytosol. Specifically, a novel clickable Ca2+ indicator "N3-fura-2 AM" was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca2+ oscillation was observed for an extended period of time.
