52571-71-8 Usage
Description
3-Nitrophenyl-alpha-D-Galactopyranoside is a chemical compound that is frequently utilized in scientific research as a substrate in enzyme assays. It is specifically reactive with certain enzymes, such as alpha-galactosidase, and upon reaction, it releases a yellow-colored product. This property allows for the quantitative measurement of enzyme activity, making it a valuable tool in various areas of biochemistry, including genetic disease research, drug development, and other studies focused on enzyme activity. Its chemical formula is C12H15NO9, and it is known to carry risks, such as causing severe eye irritation and potentially respiratory irritation.
Uses
Used in Biochemical Research:
3-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE is used as a substrate in enzyme assays for the purpose of measuring enzyme activity. Its reaction with alpha-galactosidase results in a yellow-colored product, which can be quantitatively measured, making it an essential tool in determining the activity of various enzymes.
Used in Genetic Disease Research:
3-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE is used as a diagnostic tool for genetic diseases that involve enzyme deficiencies or abnormalities. By assessing the activity of specific enzymes, researchers can gain insights into the underlying mechanisms of these diseases and develop potential therapeutic strategies.
Used in Drug Development:
3-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE is used as a screening agent in the development of new drugs. Its ability to measure enzyme activity can help in identifying potential drug candidates that can modulate enzyme activity, thus offering a pathway for the treatment of various diseases.
Used in Enzyme Activity Studies:
3-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE is used as a research compound in studies that aim to understand the mechanisms of enzyme action, regulation, and inhibition. Its reactivity with specific enzymes provides a means to investigate the interactions between enzymes and their substrates, as well as the effects of various factors on enzyme activity.
Check Digit Verification of cas no
The CAS Registry Mumber 52571-71-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,2,5,7 and 1 respectively; the second part has 2 digits, 7 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 52571-71:
(7*5)+(6*2)+(5*5)+(4*7)+(3*1)+(2*7)+(1*1)=118
118 % 10 = 8
So 52571-71-8 is a valid CAS Registry Number.
52571-71-8Relevant articles and documents
Strong Inhibition of Cholera Toxin B Subunit by Affordable, Polymer-Based Multivalent Inhibitors
Haksar, Diksha,De Poel, Eyleen,Van Ufford, Linda Quarles,Bhatia, Sumati,Haag, Rainer,Beekman, Jeffrey,Pieters, Roland J.
, p. 785 - 792 (2019/02/05)
Cholera is a potentially fatal bacterial infection that affects a large number of people in developing countries. It is caused by the cholera toxin (CT), an AB5 toxin secreted by Vibrio cholera. The toxin comprises a toxic A-subunit and a pentameric B-subunit that bind to the intestinal cell surface. Several monovalent and multivalent inhibitors of the toxin have been synthesized but are too complicated and expensive for practical use in developing countries. Meta-nitrophenyl α-galactoside (MNPG) is a known promising ligand for CT, and here mono- and multivalent compounds based on MNPG were synthesized. We present the synthesis of MNPG in greatly improved yields and its use while linked to a multivalent scaffold. We used economical polymers as multivalent scaffolds, namely, polyacrylamide, dextran, and hyperbranched polyglycerols (hPGs). Copper-catalyzed alkyne azide cycloaddition reaction (CuAAC) produced the inhibitors that were tested in an ELISA-type assay and an intestinal organoid swelling inhibition assay. The inhibitory properties varied widely depending on the type of polymer, and the most potent conjugates showed IC50 values in the nanomolar range.