333338-18-4

Basic information

• Product Name: 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
• Synonyms: PNP-PHOS DINA HEXAHYDRATE;PNPP DISODIUM HEXAHYDRATE;PNPP DISODIUM SALT HEXAHYDRATE;PHOSPHATASE SUBSTRATE;PHOSPHATASE SUBSTRATE DISODIUM SALT HEXAHYDRATE;p-Nitrophenyl phosphate disodium hexahydrate ,98%;Disodium 4-Nitrophenyl Phosphate Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt;SodiuM 4-nitrophenyl phosphate hexahydrate
• CAS NO: 333338-18-4
• Molecular Formula: C6H16NNa2O12P
• Molecular Weight: 371.14
• EINECS: 224-246-5
• Product Categories: substrate
• Mol File: 333338-18-4.mol
• Article Data: 0

Chemical Properties

• Melting Point: >300 °C(lit.)
• Boiling Point: 457.8°Cat760mmHg
• Flash Point: 230.7°C
• Appearance: white to pale yellow/tablets
• Density: g/cm³
• Vapor Pressure: 3.55E-09mmHg at 25°C
• Storage Temp.: 2-8°C
• Solubility: H2O: 25 mg/mL at 20 °C, clear, yellow-gree
• Water Solubility: almost transparency
• BRN: 5324661
• CAS DataBase Reference: 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE(CAS DataBase Reference)
• NIST Chemistry Reference: 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE(333338-18-4)
• EPA Substance Registry System: 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE(333338-18-4)

Safety Data

• Safety Statements: 24/25
• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 333338-18-4(Hazardous Substances Data)

Usage

Description

4-Nitrophenyl phosphate disodium salt hexahydrate is a synthetic chemical compound that serves as a chromogenic substrate in various biochemical assays. It is characterized by its ability to produce a color change upon enzymatic hydrolysis, which allows for the detection and quantification of specific enzymes, such as acid and alkaline phosphatases.

Uses

Used in Enzyme Assays:
4-Nitrophenyl phosphate disodium salt hexahydrate is used as a substrate for the determination of acid and alkaline phosphatases. Upon enzymatic hydrolysis, it releases 4-nitrophenolate ion, which imparts a yellow color to the solution. This color change can be measured spectrophotometrically, allowing for the quantification of enzyme activity.
Used in Diagnostic Applications:
In the field of diagnostics, 4-Nitrophenyl phosphate disodium salt hexahydrate is used in enzyme-linked immunosorbent assays (ELISA) for various purposes. It has been employed to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I), enabling the study of platelet-collagen interactions. Additionally, it is used for the semi-quantitative measurement of allergen-specific IgE by ELISA, aiding in the diagnosis of allergic reactions.
Used in Research Applications:
4-Nitrophenyl phosphate disodium salt hexahydrate serves as a substrate in alkaline phosphatase assays, which are commonly used in research settings to study enzyme kinetics, enzyme inhibition, and the effects of various factors on enzyme activity. This allows researchers to gain insights into the mechanisms of enzyme function and develop potential therapeutic agents.
Used in Quality Control:
In the context of quality control, 4-Nitrophenyl phosphate disodium salt hexahydrate has been used as a phosphatase substrate for the chromogenic detection of adsorbed rabbit IgG. This application helps determine the coating efficiency of antibodies on assay plates, ensuring the reliability and accuracy of immunoassays.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

InChI:InChI=1/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2