32860-54-1 Usage
Uses
Used in Molecular Biology and Genetics Research:
5-methylaminomethyl-2-thiouridine is used as a research tool for studying the structure and function of tRNA molecules, as well as the mechanisms of protein synthesis. Its presence in tRNAs specific for Lys and Glu makes it an important target for understanding the decoding process and the role of modified nucleosides in ensuring accurate translation of the genetic code.
Used in Drug Development:
The unique properties of 5-methylaminomethyl-2-thiouridine make it a potential target for the development of new drugs that could modulate protein synthesis. By targeting the interaction of this modified nucleoside with the ribosome or other components of the translation machinery, researchers may be able to develop novel therapeutics for various diseases, including those related to protein misfolding or aberrant protein synthesis.
Used in Biochemical and Structural Studies:
5-methylaminomethyl-2-thiouridine can be used as a probe in biochemical and structural studies to investigate the interactions between tRNA and other components of the translation machinery, such as ribosomes and aminoacyl-tRNA synthetases. This information can provide valuable insights into the molecular mechanisms underlying protein synthesis and the role of modified nucleosides in this process.
Used in Synthetic Biology:
The unique properties of 5-methylaminomethyl-2-thiouridine can also be exploited in the field of synthetic biology, where researchers engineer novel biological systems with specific functions. By incorporating this modified nucleoside into synthetic tRNAs or other RNA molecules, researchers can potentially create new ribosomes or other translation machinery components with altered or enhanced functions.
Used in Diagnostics:
5-methylaminomethyl-2-thiouridine could be used in the development of diagnostic tools for detecting specific tRNA modifications or monitoring the fidelity of protein synthesis in cells. By measuring the levels of this modified nucleoside in tRNAs, researchers may be able to gain insights into the cellular processes involved in protein synthesis and identify potential biomarkers for various diseases or conditions.
Check Digit Verification of cas no
The CAS Registry Mumber 32860-54-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,2,8,6 and 0 respectively; the second part has 2 digits, 5 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 32860-54:
(7*3)+(6*2)+(5*8)+(4*6)+(3*0)+(2*5)+(1*4)=111
111 % 10 = 1
So 32860-54-1 is a valid CAS Registry Number.
InChI:InChI=1/C11H17N3O5S/c1-12-2-5-3-14(11(20)13-9(5)18)10-8(17)7(16)6(4-15)19-10/h3,6-8,10,12,15-17H,2,4H2,1H3,(H,13,18,20)/t6-,7-,8-,10-/m1/s1
32860-54-1Relevant articles and documents
Synthesis of various substituted 5-methyluridines (xm5U) and 2-thiouridines (xm5s2U) via nucleophilic substitution of 5-pivaloyloxymethyluridine/2-thiouridine
Bartosik, Karolina,Leszczynska, Grazyna
, p. 6593 - 6597 (2015)
5-Pivaloyloxymethyluridine and its 2-thio analogue have been utilized as convenient substrates for the synthesis of various 5-methyluridines (xm5U) and 5-methyl-2-thiouridines (xm5s2U). The pivaloyloxy group (OPiv) located at the pseudobenzylic position was effectively substituted with a series of nucleophiles: ammonia, primary and secondary amines including secondary cyclic amines, tetrabutylammonium salts of amino acids, an alkoxide and a thiolate.
Chemistry and structure of modified uridines in the anticodon, wobble position of transfer RNA are determined by thiolation
Sierzputowska-Gracz,Sochacka,Malkiewicz,Kuo,Gehrke,Agris
, p. 7171 - 7177 (2007/10/07)
Uridines found in the first or wobble position of transfer RNA anticodons are most often modified at base ring carbon-5 and many times also thiolated at carbon-2. It is important to understand the chemistry and structure of the modified uridines because t
THE PROTECTED DERIVATES OF 5-METHYLAMINOMETHYL-2-THIOURIDINE AND 5-CARBOMETHOXYMETHYL-2-THIOURIDINE AS COMPONENTS FOR THE OLIGONUCLEOTIDE SYNTHESIS
Malkiewicz, A.,Sochacka, E.
, p. 5387 - 5390 (2007/10/02)
The syntheses of protected derivates of 5-methylaminomethyl-2-thiouridine(mnm5s2U) 11, 12 and 5-carbomethoxymethyl-2-thiouridine(mcm5s2U) 13 as well as their unprotected 3'-phosphates 14, 15 have been described.
Nucleoside Syntheses, XXI. Synthesis of 5-Methylaminomethyl-2-thiouridine, a Rare Nucleoside from t-RNA
Vorbrueggen, Helmut,Krolikiewicz, Konrad
, p. 1438 - 1447 (2007/10/02)
The synthesis of 5-methylaminomethyl-2-thiouridine (1), a rare nucleoside from t-RNA, starting from the appropriate 2-thiouracil and ribose derivatives is described.During the silyl Hilbert-Johnson nucleoside synthesis with silver perchlorate the tert-butoxycarbonyl (Boc) aminoprotecting group in the 2-thiouracil moiety is removed.This cleavage is due to the intermediate formation of trimethylsilyl perchlorate.Preparative applications of this new type of Lewis acids are mentioned.