25389-94-0 Usage
Description
Kanamycin sulfate is a broad-spectrum aminoglycoside antibiotic derived from Streptomyces kanamyceticus. It is known for its effective action against serious infections caused by gram-negative bacteria and is used as an additive in culture media for various applications.
Uses
Used in Pharmaceutical Industry:
Kanamycin sulfate is used as an aminoglycoside antibiotic for treating infections caused by sensitive bacteria, including pulmonary infection, urinary tract infection, biliary tract infection, sepsis, and abdominal cavity infection. It has demonstrated effective antibacterial activity in the treatment of acute gonorrheal urethritis in men and has been found valuable in treating serious infections in infants and children.
Used in Tissue Culture Media:
Kanamycin sulfate is used as a selection agent in tissue culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for the selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. It is also used as a selection agent for cells transformed with the kanamycin B resistance gene, with a recommended concentration of 100mg/mL for cell culture applications.
Used in Isolation Media:
Kanamycin monosulfate is used as a selection agent in several types of isolation media, aiding in the growth and selection of specific bacterial strains.
Used in Antibacterial Applications:
Kanamycin sulfate is an antibacterial agent similar to neomycin, providing broad-spectrum coverage against gram-negative bacteria such as Escherichia coli, Klebsiella, Proteus, Pneumonia, Enterobacter aerogenes, and Shigella.
Chemical Properties:
Kanamycin sulfate is an off-white powder with potent antibiotic properties.
Reference
JE Fischnaller; AH Pedersen; AR Ronald; P Bonin; Tronca, E., Kanamycin sulfate in the treatment of acute gonorrheal urethritis in men. The Journal of the American Medical Association 1968, 203, 909-912.
MD Yow; Tengg, N., The use of kanamycin sulfate as a therapeutic agent for infants and children. An analysis of 140 cases treated during a two-year period. Journal of Pediatrics 1961, 58, 538-547.
Originator
Kantrex,Bristol,US,1958
Manufacturing Process
As described in US Patent 2,931,798, Streptomyces kanamyceticus (K2-J) was
first cultured in shake flasks in the following media: (a) 0.75% meat extract,
0.75% peptone, 0.3% NaCl, with 1.0% of starch, dextrin, maltose, glucose,
lactose, sucrose or glycerol; or (b) 2.0% soybean meal, 0.05% KCl, 0.05%
MgSO4 · 7H2O, 0.5% NaCl, 0.2% NaNO3, with 1.0% of starch, dextrin,
maltose, glucose, lactose, sucrose or glycerol. The initial pH of all media was
adjusted to 7.0. After 24 to 48 hours shaking in some cases the pH decreased
to about 6.0 to 6.8, but from 72 to 120 hours the pH rose and became 7.5 to
8.6. The production of kanamycin was apparent after 48 hours and, depending
on the media; the maximum production was found after 72 to 120 hours.The yield was highest with starch or dextrin, intermediate and about the same
with sucrose, glucose, maltose and lactose and poorest with glycerol.
Kanamycin was produced by media containing soybean meal, peanut meal,
cottonseed meal, corn steep liquor, peptone, yeast extract or meat extract,
with or without sodium nitrate. Commercially available soybean meal was
recognized to be one of the best nitrogen sources. The addition of corn steep
liquor, peptone, yeast extract or nitrate to the soybean meal promoted the
production of kanamycin.The brownish white kanamycin (5 g) was dissolved in 50 ml of 60% aqueous
methanol, insoluble material was removed and to the filtrate 40 ml of 60%
aqueous methanol containing 2,000 mg of ammonium sulfate was added, and
the precipitated kanamycin sulfate was collected, washed with 50 ml of 80%
aqueous methanol, and dried. Thus, 4.5 g of kanamycin sulfate was obtained
as a light brownish powder.
Therapeutic Function
Antibacterial
Biological Activity
soluble in water at 50mg/ml recommend our ultra pure, nuclease free water, ab02123 typically used at 100mg/ml for cell culture protect from moisture store: 2-8°c.
Biochem/physiol Actions
Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding. Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.
Check Digit Verification of cas no
The CAS Registry Mumber 25389-94-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,5,3,8 and 9 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 25389-94:
(7*2)+(6*5)+(5*3)+(4*8)+(3*9)+(2*9)+(1*4)=140
140 % 10 = 0
So 25389-94-0 is a valid CAS Registry Number.
25389-94-0Relevant articles and documents
The last step of kanamycin biosynthesis: Unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK
Sucipto, Hilda,Kudo, Fumitaka,Eguchi, Tadashi
supporting information; experimental part, p. 3428 - 3431 (2012/06/30)
Mystery solved: Using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism (see scheme) to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered. Copyright