24611-43-6Relevant articles and documents
Copper complex of a thienyl-hydrazone rhodamine derivative is a highly selective colorimetric sensor for pyrophosphate
Kumar, Pradeep,Kumara, Guralamatta Siddappa Ravi,Seo, Young Jun
supporting information, (2022/01/08)
Detection of pyrophosphate (PPi) can be used to monitor gene replication, transcription, and other forms of enzymatic DNA/RNA synthesis, but it can be difficult to perform in real samples because of the presence of many other biomolecules and buffer components. In this study we prepared a colorimetric probe that could detect PPi specifically and selectively in the presence of cysteine, dithiothreitol (DTT), nucleoside triphosphates, and human serum (containing several biomolecules and amino acids). Among four candidate rhodamine derivatives, we found that the copper complex 1 could detect PPi uniquely, even in the presence of several competing biomolecules. The copper complex 1 also exhibited its highly selective PPi sensing capability when applied to rolling circle amplification and in vitro transcription in samples containing high concentrations of DTT, making it a promising candidate for colorimetric detection of RNA/DNA when using amplification techniques.
Synthesis of some 1,3,4-thiadiazole derivatives as inhibitors of Entamoeba histolytica
Siddiqui, Shadab Miyan,Salahuddin, Attar,Azam, Amir
, p. 1305 - 1312 (2013/04/10)
In the quest for potent anti-amoebic agents, some 1,3,4-thiadiazole derivatives were synthesized and characterized by spectral data. The purity of the compounds was confirmed by elemental analysis. All the compounds were screened in vitro against HM1:IMSS strain of Entamoeba histolytica by microdilution method. The results revealed that compounds 1 (IC50 = 0.670 μM), 3 (IC50 = 1.60 μM) and 8 (IC50 = 0.522 μM) had much better anti-amoebic activity than the reference drug metronidazole (IC50 = 1.80 μM). Further, cytotoxicity of the compounds having IC50 value less than metronidazole was assessed by MTT assay on human breast cancer MCF-7 cell line and all the compounds were found low cytotoxic in the concentration range of 2.5-250 μM. Preliminary results indicate that these three compounds (1, 3 and 8) may be subjected to further investigations and it may be hoped that the present study will stimulate efforts towards the development of novel effective anti-amoebic agents.
Condensed 1,3-Benzothiazinones. 1. Facile Synthesis of 2-Amino-1,2,4-triazolobenzothiazin-9-one
Liu, Kang-Chien,Shih, Bi-Jane,Chern, Ji-Wang
, p. 1215 - 1217 (2007/10/02)
Treating 2-mercaptobenzohydrazide (1a) with cyanogen bromide gave 3-amino-2-imino-3,4-dihydro-2H-1,3-benzothiazin-4-one (2a).This compound underwent further cyclocondensation with a second molecule of cyanogen bromide or S-methylthiourea sulfate to afford