216173-04-5Relevant articles and documents
In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe
Chen, Ji-An,Fu, Wei,Gu, Xianfeng,Guo, Wei,Hu, Wei,Ouyang, Zhirong,Pan, Hongming,Sun, Nannan,Tan, Jiahui,Wang, Yonghui,Wang, Zhijun
, p. 12613 - 12621 (2020)
Senescence-associated diseases have severely diminished the quality of life and health of patients. However, a sensitive assay of these diseases remains limited due to a lack of straightforward methods. Considering that senescence-associated β-galactosidase (SA-β-Gal) is overexpressed in senescent cells, the detection of SA-β-Gal in senescent cells and tissues might be a feasible strategy for the early diagnosis of SA diseases. In this study, a β-galactosidase-activatable nanoprobe BOD-L-βGal-NPs was developed for the imaging of senescent cells and vasculature in atherosclerotic mice via real-time monitoring of β-Gal. BOD-L-βGal-NPs was fabricated by encapsulating a newly designed NIR ratiometric probe BOD-L-βGal within a poly(lactic-co-glycolic) acid (PLGA) core. Nanoprobe BOD-L-βGal-NPs showed good accumulation in arteries, thus successfully visualizing senescent cells and vasculature in atherosclerotic mice by tail vein injection. Our findings indicated that nanoprobe BOD-L-βGal-NPs holds great potential for the early diagnosis and therapy of atherosclerosis and other aging-associated diseases.
Exploring the differential recognition of DNA G-quadruplex targets by small molecules using dynamic combinatorial chemistry
Bugaut, Anthony,Jantos, Katja,Wietor, Jean-Luc,Rodriguez, Raphael,Sanders, Jeremy K. M.,Balasubramanian, Shankar
, p. 2677 - 2680 (2008/12/23)
Tuning affinities: Dynamic combinatorial chemistry has been employed to explore the effect of chemical modifications on the DNA G-quadruplex binding properties of anoxazole-based peptide macrocycle. Two dynamic libraries of molecules, containing cationic and carbohydrate motifs, respectively, were screened by using structurally diverse nucleic acid targets (see scheme; each colored shape represents a cation or carbohydrate). (Chemical Equation Presented).