2044-85-1 Usage
Description
2',7'-DICHLOROFLUORESCIN DIACETATE (DCDHF) is a cell-permeable, sensitive indicator of peroxynitrite formation. It is a derivative of fluorescein, a synthetic organic compound commonly used as a fluorescent dye. DCDHF is utilized in various applications due to its unique properties, such as its ability to be hydrolyzed by cytosolic esterases or through base-catalyzed cleavage of the diacetate groups, followed by oxidation by peroxynitrite to yield a highly fluorescent product.
Uses
Used in Cellular Biology and Oxidative Stress Research:
2',7'-DICHLOROFLUORESCIN DIACETATE is used as a fluorescent probe for detecting peroxynitrite formation in cells. This application is crucial for understanding the role of reactive nitrogen species in various biological processes and diseases.
Used in Reactive Oxygen Species (ROS) Quantification:
2',7'-DICHLOROFLUORESCIN DIACETATE is used as a component/substrate of the 2',7'-dichlorofluorescein diacetate assay to quantitate reactive oxygen species (ROS). This assay is essential for studying oxidative stress and its implications in various pathological conditions.
Used in Environmental and Analytical Chemistry:
2',7'-DICHLOROFLUORESCIN DIACETATE may be used as a superior gas phase alternative to underivatized fluorescein. This application allows for the sensitive detection and quantification of peroxynitrite and other reactive species in environmental and analytical chemistry settings.
Check Digit Verification of cas no
The CAS Registry Mumber 2044-85-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,0,4 and 4 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 2044-85:
(6*2)+(5*0)+(4*4)+(3*4)+(2*8)+(1*5)=61
61 % 10 = 1
So 2044-85-1 is a valid CAS Registry Number.
2044-85-1Relevant articles and documents
Electronic and Steric Optimization of Fluorogenic Probes for Biomolecular Imaging
Chyan, Wen,Kilgore, Henry R.,Gold, Brian,Raines, Ronald T.
, p. 4297 - 4304 (2017/04/28)
Fluorogenic probes are invaluable tools for spatiotemporal investigations within live cells. In common fluorogenic probes, the intrinsic fluorescence of a small-molecule fluorophore is masked by esterification until entry into a cell, where endogenous esterases catalyze the hydrolysis of the masking groups, generating fluorescence. The susceptibility of masking groups to spontaneous hydrolysis is a major limitation of these probes. Previous attempts to address this problem have incorporated auto-immolative linkers at the cost of atom economy and synthetic adversity. Here, we report on a linker-free strategy that employs adventitious electronic and steric interactions in easy-to-synthesize probes. We find that X···C = O n→π? interactions and acyl group size are optimized in 2′,7′-dichlorofluorescein diisobutyrate. This probe is relatively stable to spontaneous hydrolysis but is a highly reactive substrate for esterases both in vitro and in cellulo, yielding a bright, photostable fluorophore with utility in biomolecular imaging.
TUMOR NECROSIS FACTOR INHIBITORS
-
Page/Page column 43; 46, (2008/06/13)
The present invention is directed to compounds that are allosteric inhibitors of tumor necrosis factor receptor I, compositions comprising such compounds, and methods of using such compounds and compositions thereof in the treatment of TNF-α mediated conditions.
