193967-71-4Relevant articles and documents
DOTA-amide lanthanide tag for reliable generation of pseudocontact shifts in protein NMR spectra
Graham, Bim,Loh, Choy Theng,Swarbrick, James David,Ung, Phuc,Shin, James,Yagi, Hiromasa,Jia, Xinying,Chhabra, Sandeep,Barlow, Nicholas,Pintacuda, Guido,Huber, Thomas,Otting, Gottfried
, p. 2118 - 2125 (2011)
Structural studies of proteins and protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy can be greatly enhanced by site-specific attachment of lanthanide ions to create paramagnetic centers. In particular, pseudocontact shifts (PCS) g
A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time-Resolved Luminescence Studies
Herath, Iresha D.,Breen, Colum,Hewitt, Sarah H.,Berki, Thomas R.,Kassir, Ahmad F.,Dodson, Charlotte,Judd, Martyna,Jabar, Shereen,Cox, Nicholas,Otting, Gottfried,Butler, Stephen J.
supporting information, p. 13009 - 13023 (2021/08/03)
A lanthanide-binding tag site-specifically attached to a protein presents a tool to probe the protein by multiple spectroscopic techniques, including nuclear magnetic resonance, electron paramagnetic resonance and time-resolved luminescence spectroscopy. Here a new stable chiral LnIII tag, referred to as C12, is presented for spontaneous and quantitative reaction with a cysteine residue to generate a stable thioether bond. The synthetic protocol of the tag is relatively straightforward, and the tag is stable for storage and shipping. It displays greatly enhanced reactivity towards selenocysteine, opening a route towards selective tagging of selenocysteine in proteins containing cysteine residues. Loaded with TbIII or TmIII ions, the C12 tag readily generates pseudocontact shifts (PCS) in protein NMR spectra. It produces a relatively rigid tether between lanthanide and protein, which is beneficial for interpretation of the PCSs by single magnetic susceptibility anisotropy tensors, and it is suitable for measuring distance distributions in double electron–electron resonance experiments. Upon reaction with cysteine or other thiol compounds, the TbIII complex exhibits a 100-fold enhancement in luminescence quantum yield, affording a highly sensitive turn-on luminescence probe for time-resolved FRET assays and enzyme reaction monitoring.
Value of zeolites in asymmetric induction during photocyclization of pyridones, cyclohexadienones and naphthalenones
Sivasubramanian, Karthikeyan,Kaanumalle, Lakshmi S.,Uppili, Sundararajan,Ramamurthy
, p. 1569 - 1576 (2008/02/05)
Two strategies, namely chiral inductor and chiral auxiliary approaches, have been examined within zeolites with the aim of achieving asymmetric induction during the photocyclization of cyclohexadienone, naphthalenone and pyridone derivatives. Within zeoli