119736-16-2Relevant articles and documents
Purification and characterization of molybdenum-containing aldehyde dehydrogenase that oxidizes benzyl maltol derivative from Pseudomonas nitroreducens SB32154
Hibi, Makoto,Kozono, Iori,Ogawa, Jun,Takeuchi, Michiki
, p. 2390 - 2400 (2020)
Maltol derivatives are used in a variety of fields due to their metal-chelating abilities. In the previous study, it was found that cytochrome P450 monooxygenase, P450nov, which has the ability to effectively convert the 2-methyl group in a maltol derivative, transformed 3-benzyloxy-2-methyl-4-pyrone (BMAL) to 2-(hydroxymethyl)-3-(phenylmethoxy)-4H-pyran-4-one (BMAL-OH) and slightly to 3-benzyloxy-4-oxo-4 H-pyran-2-carboxaldehyde (BMAL-CHO). We isolated Pseudomonas nitroreducens SB32154 with the ability to convert BMAL-CHO to BMAL-COOH from soil. The enzyme responsible for aldehyde oxidation, a BMAL-CHO dehydrogenase, was purified from P. nitroreducens SB32154 and characterized. The purified BMAL-CHO dehydrogenase was found to be a xanthine oxidase family enzyme with unique structure of heterodimer composed of 75 and 15 kDa subunits containing a molybdenum cofactor and [Fe-S] clusters, respectively. The enzyme showed broad substrate specificity toward benzaldehyde derivatives. Furthermore, one-pot conversion of BMAL to BMAL-COOH via BMAL-CHO by the combination of the BMAL-CHO dehydrogenase with P450nov was achieved.
Method for preparing baloxavir intermediate
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Paragraph 0039; 0046-0049; 0056-0059; 0066-0068, (2021/01/11)
The invention belongs to the technical field of chemical synthesis and provides a method for preparing a balosavir intermediate. According to the method disclosed by the invention, cheap and easily available 3-hydroxy-2-(hydroxymethyl)-4H-pyran-4-ketone (a compound shown as a formula I) is used as a starting material; TEMPO oxidation, benzyl protection and hydrolysis reaction are carried out; so that the intermediate compound---3-benzyloxy-4-oxo-4H-pyran-2-formic acid shown as a formula Baloxavir is prepared. According to the method, highly toxic chemicals such as methylsulfonyl chloride and selenium dioxide are prevented from being used, and reaction reagents are common materials. In each step of the method, the temperature is controlled below 60 DEG C, high-temperature reaction is avoided, and the safety coefficient is high. The method has the advantages of short synthesis route, short reaction steps, simple operation, common and easily purchased reagents and low price, and suitability for industrial production.
Preparation method of pyrone compound
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Paragraph 0042; 0051-0054, (2020/09/23)
The invention provides a preparation method of a pyrone compound, and belongs to the field of pharmaceutical chemicals. According to the method, a pyrone compound sequentially reacts with acetal, concentrated sulfuric acid and sodium chlorite respectively, and extraction is performed to obtain a high-purity pyrone compound. The product produced by the method has the characteristics of high purity,high yield, low cost, simple operation and stable process.