Chemical Science
ARTICLE
DOI: 10.1039/C6SC02842J
Journal Name
one can expect that ER based process could lead to similar high 11. N. Z. Xie, H. Liang, R. B. Huang and P. Xu, Biotechnol. Adv., 2014, 32,
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titers. In particular, ERꢀBC exhibits high oxygen tolerance and
thermostability making it useful for in vivo and in vitro
applications to overcome limitations of chemical catalysts.
Thus, the C=C hydrogenation activity of ERs demonstrated in
our work can potentially replace the chemical hydrogenation
step in current synthetic protocols creating a completely bioꢀ
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Acknowledgements
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The authors thank all members of the BioZone Centre for Applied 17. H.ꢀM. Jung, M.ꢀY. Jung and M.ꢀK. Oh, Appl. Microbiol. Biotechnol.,
Science and Bioengineering and Christine Achampong for help in
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conducting the experiments. Dr. K. T. Shanmugam (University of 18. D. R. Vardon, N. A. Rorrer, D. Salvachua, A. E. Settle, C. W. Johnson,
M. J. Menart, N. S. Cleveland, P. N. Ciesielski, K. X. Steirer, J. R.
Dorgan and G. T. Beckham, Green Chem., 2016, 18, 3397ꢀ3413.
9. D. R. Vardon, M. A. Franden, C. W. Johnson, E. M. Karp, M. T.
Guarnieri, J. G. Linger, M. J. Salm, T. J. Strathmann and G. T. Beckham,
Energ. Envrion. Sci., 2015, 8, 617ꢀ628.
Florida) and Dr. P. Hallenbeck (University of Montreal) are thanked
for providing the B. coagulans 36D1 genomic DNA and the E. coli
BL21 (DE3) ∆iscR strain, respectively. This work was supported by
the Government of Canada through Genome Canada and the Ontario
Genomics Institute (2009ꢀOGIꢀABCꢀ1405), Ontario Research Fund
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polymerization of Bioꢀpolyamide 510) funded by the Ministry of
Trade, Industry & Energy (MOTEI, Korea).
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These authors contributed equally to this work.
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