1
,25(OH) D INCREASES PMCA1b ACTIVITY AND EXPRESSION IN OSTEOBLASTS
533
2
3
for the role of 1,25(OH) D in the maintenance of calcium 15. Meszaros JG, Karin NJ 1993 Osteoblasts express the PMCA1b
2
3
2
ϩ
isoform of the plasma membrane Ca -ATPase. J Bone Miner
homeostasis.
Res 8:1235–1240.
ϩ
2ϩ
1
1
1
6. White KE, Gesek FA, Friedman PA 1996 Na /Ca exchange
in rat osteoblast-like UMR 106 cells. J Bone Miner Res 11:
1
666–1675.
ACKNOWLEDGMENTS
7. Laemmli UK 1970 Cleavage of structural proteins during the
assembly of the head of bacteriophage T4. Nature 227:680–
P.G. was supported by the National Health and Medical
Research Council of Australia (NHMRC) Medical Post-
graduate Scholarship (987643) and the University of West-
ern Australia, Athelstan and Amy Saw Foundation Stipend.
I.D. was the recipient of the NHMRC Australian Biomed-
6
85.
8. Towbin H, Staehelin T, Gordon J 1979 Electrophoretic trans-
fer of proteins from polyacrylamide gels to nitrocellulose
sheets: Procedure and some applications. Proc Natl Acad Sci
USA 76:4350–4354.
ical Postgraduate Research Scholarship (987528). This 19. Stauffer TP, Guerini D, Carafoli E 1995 Tissue distribution of
2
ϩ
project was supported by a grant from the Sir Charles
Gairdner Hospital Research Foundation.
the four gene products of the plasma membrane Ca pump. A
study using specific antibodies. J Biol Chem 270:12184–
1
2190.
2
0. Hilfiker H, Strehler-Page MA, Stauffer TP, Carafoli E,
Strehler EE 1993 Structure of the gene encoding the human
plasma membrane calcium pump isoform 1. J Biol Chem
REFERENCES
2
68:19717–19725.
2
2
1. Zelinski JM, Sykes DE, Weiser MM 1991 The effect of
vitamin D on rat intestinal plasma membrane Ca-pump
mRNA. Biochem Biophys Res Commun 179:749–755.
1
2
. Miller SC, Jee WS 1987 The bone lining cell: A distinct
phenotype? Calcif Tissue Int 41:1–5.
. Hillsley MV, Frangos JA 1996 Osteoblast hydraulic conduc-
tivity is regulated by calcitonin and parathyroid hormone.
J Bone Miner Res 11:114–124.
2. Cai Q, Chandler JS, Wasserman RH, Kumar R, Penniston JT
1
993 Vitamin D and adaptation to dietary calcium and phos-
phate deficiencies increase intestinal plasma membrane cal-
cium pump gene expression. Proc Natl Acad Sci USA 90:
3
. Shen V, Hruska K, Avioli LV 1988 Characterization of a
2
ϩ
2ϩ
(Ca
ϩ Mg )-ATPase system in the osteoblast plasma
1
345–1349.
membrane. Bone 9:325–329.
2
3. Gay E, Babajko S 2000 AUUUA sequences compromise hu-
man insulin-like growth factor binding protein-1 mRNA sta-
bility Biochem Biophys Res Commun 267:509–515.
4. Enomoto H, Hendy GN, Andrews GK, Clemens TL 1992
Regulation of avian calbindin-D28K gene expression in pri-
mary chick kidney cells: Importance of posttranscriptional
mechanisms and calcium ion concentration. Endocrinology
130:3467–3474.
4
. Borke JL, Eriksen EF, Minami J, Keeting P, Mann KG, Pen-
niston JT, Riggs BL, Kumar R 1988 Epitopes of the human
2
ϩ
2ϩ
erythrocyte Ca -Mg ATPase pump in human osteoblast-
2
like cell plasma membranes. J Clin Endocrinol Metab 67:
1
299–1304.
5
6
7
8
. Kumar R, Haugen JD, Penniston JT 1993 Molecular cloning of
a plasma membrane calcium pump from human osteoblasts.
J Bone Miner Res 8:505–513.
. Akisaka T, Yamamoto T, Gay CV 1988 Ultracytochemical 25. Carafoli E, Genazzani A, Guerini D 1999 Calcium controls the
investigation of calcium-activated adenosine triphosphatase
transcription of its own transporters and channels in develop-
ing neurons. Biochem Biophys Res Commun 266:624–632.
2
ϩ
(
Ca -ATPase) in chick tibia. J Bone Miner Res 3:19–25.
. Gay CV, Lloyd QP 1995 Characterization of calcium efflux by 26. Zacharias DA, Strehler EE 1996 Change in plasma membrane
2
ϩ
osteoblasts derived from long bone periosteum. Comp Bio-
chem Physiol 111:257–261.
Ca -ATPase splice-variant expression in response to a rise in
2ϩ
intracellular Ca . Curr Biol 6:1642–1652.
. Glendenning P, Ratajczak T, Dick IM, Prince RL 2000 Cal- 27. Borke JL, Minami J, Verma A, Penniston JT, Kumar R 1987
citriol upregulates expression and activity of the 1b isoform of
the plasma membrane calcium pump in immortalised distal
kidney tubular cells. Arch Biochem Biophys 380:126–132.
. Armbrecht HJ, Boltz MA, Kumar VB 1999 Intestinal plasma
Monoclonal antibodies to human erythrocyte membrane
ϩϩ ϩϩ
Ca -Mg
adenosine triphosphatase pump recognize an
epitope in the basolateral membrane of human kidney distal
tubule cells. J Clin Invest 80:1225–1231.
9
membrane calcium pump protein and its induction by 28. Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT
1
,25(OH) D decrease with age. Am J Physiol 277:G41–G47.
1996 Detection of isoform 4 of the plasma membrane calcium
pump in human tissues by using isoform-specific monoclonal
antibodies. Biochem J 316:353–359.
2
3
1
0. Glendenning P, Ratajczak T, Prince RL, Garamszegi N,
Strehler EE 2000 The promoter region of the human PMCA1
gene mediates transcriptional downregulation by 1,25- 29. Carafoli E 1994 Biogenesis: Plasma membrane calcium AT-
dihydroxyvitamin D . Biochem Biophys Res Commun 277:
7
Pase: 15 years of work on the purified enzyme. FASEB J
8:993–1002.
3
22–728.
1
1
1
1. Parys JB, De Smedt H, Borghgraef R 1986 Calcium transport 30. Clemens TL, Garrett KP, Zhou XY, Pike JW, Haussler MR,
systems in the LLC-PK1 renal epithelial established cell line.
Biochim Biophys Acta 888:70–81.
2. Chomczynski P, Sacchi N 1987 Single-step method of RNA
Dempster DW 1988 Immunocytochemical localization of the
1,25-dihydroxyvitamin D receptor in target cells. Endocrinol-
3
ogy 122:1224–1230.
isolation by acid guanidinium thiocyanate-phenol-chloroform 31. McCabe LR, Banerjee C, Kundu R, Harrison RJ, Dobner PR,
extraction. Anal Biochem 162:156–159.
3. Shull GE, Greeb J 1988 Molecular cloning of two isoforms of
Stein JL, Lian JB, Stein GS 1996 Developmental expression
and activities of specific fos and jun proteins are functionally
related to osteoblast maturation: Role of Fra-2 and Jun D
during differentiation. Endocrinology 137:4398–4408.
2
ϩ
the plasma membrane Ca -transporting ATPase from rat
brain. Structural and functional domains exhibit similarity to
ϩ
ϩ
Na , K - and other cation transport ATPases. J Biol Chem 32. Zacharias DA, Kappen C 1999 Developmental expression of
2
63:8646–8657.
the four plasma membrane calcium ATPase (Pmca) genes in
1
4. Criddle RA, Zheng MH, Dick IM, Callus B, Prince RL 1997
the mouse. Biochim Biophys Acta 1428:397–405.
Estrogen responsiveness of renal calbindin-D28K gene expres- 33. Freeman TC, Howard A, Bentsen BS, Legon S, Walters JR
sion in rat kidney. J Cell Biochem 65:340–438. 1995 Cellular and regional expression of transcripts of the