α-GALACTOSIDASE OF POTERIOOCHROMONAS MALHAMENSIS

Article abstract of DOI:10.1016/0031-9422(81)85216-8

Key Word Index - Poterioochromonas malhamensis; alga; α-galactosidase; isofloridoside; osmotic regulation; stability. In cell-free extracts of Poterioochromonas malhamensis, α-galactosidase α-D-galactoside galactohydrolase, EC 3.2.1.22) was stable at pH 8.The pH optimum of the enzyme was 7.The enzyme was purified 10-fold through chromatographic steps, involving DEAE-cellulose, hydroxyapatite and Sephadex G-200.The apparent MW was 360 000 by sucrose density-gradient centrifugation.All activity was lost on subjecting the enzyme to polyacrylamide-gel electrophoresis.The substrate specificity of the enzyme was examined and some kinetic values determined.The enzyme displayed an unusual activity curve with respect to isofloridoside.