10.1002/chem.202003140
Chemistry - A European Journal
FULL PAPER
The biocatalytic reaction was carried out in an ammonium formate buffer
(0.5 mL, 2 M, pH 8.5) by adding NAD+ (1 mM), Cb-FDH (16 μM), LE-AmDH
(90 μM) and ketone (10 mM). The reaction was incubated at 50 °C in an
orbital shaker (170 rpm) for 48 h. Then the reaction was basified with KOH
(100 μL, 10 M) and extracted with EtOAc (1 x 600 μL). The organic phase
was dried with MgSO4 and analyzed by GC-FID. For details, see SI,
section 5.
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General procedure for the reduction of ketones and aldehydes to
alcohols
The biocatalytic reaction was carried out in a potassium phosphate buffer
(1 mL, 100 mM, pH 7) supplemented with sodium formate (100 mM) by
adding NAD+ (1 mM), Cb-FDH (16 μM), LE-AmDH (45 μM) and aldehyde
or ketone (20 mM) in consecutive order. The reaction was incubated at
30 °C in an orbital shaker (170 rpm) for 24 h. Then the reaction was
extracted with EtOAc (2 x 500 μL, containing internal standard). The
combined organic phase was dried with MgSO4 and analyzed by GC-FID.
For details, see SI, section 5.
One-enzyme conversion of benzylic alcohol (1a) to benzylamine (1c)
The biocatalytic reaction was carried out in a Tris-HCl buffer (1 mL, 100
mM) supplemented with NH4OH (1 M) at a final pH value of 9 and by
adding NAD+ (1 mM), LE-AmDH (90 μM) and 1a (10 mM). The reaction
was incubated at 30 °C in an orbital shaker (170 rpm) for 48 h. Then the
reaction was basified with KOH (200 μL, 10 M) and extracted with EtOAc
(2 x 500 μL, containing internal standard). The combined organic phase
was dried with MgSO4 and analyzed by GC-FID. For details, see SI,
section 5.
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This project has received funding from the European Research
Council (ERC) under the European Union’s Horizon 2020
research and innovation programme (ERC-StG, grant agreement
No 638271, BioSusAmin). Dutch funding from the NWO Sector
Plan for Physics and Chemistry is also acknowledged.
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Keywords: Biocatalysis • amine dehydrogenases • alcohol
dehydrogenases • alcohol amination • enzyme promiscuity
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