- Method for recovering kanamycin A from amikacin synthesis solution
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The invention discloses a method for recovering kanamycin A from an amikacin synthesis solution. The amikacin synthesis solution mainly comprises K29, kanamycin A and amikacin. The method for recovering kanamycin A comprises the following steps: 1) purifying the amikacin synthesis solution through a macro-porous cation exchange resin to obtain kanamycin A, amikacin and K29 respectively; 2) hydrolyzing the K29, obtained by purification in step 1), by using an alkali to obtain kanamycin A; and 3) mixing the kanamycin A obtained in the step 1) with the kanamycin A obtained in step 2). The methodallows a part of kanamycin A to be directly recovered from the amikacin synthesis solution, and allows the remaining kanamycin A to be recovered by simple synthesis steps of other recycled components,so the cost is greatly reduced, a reaction waste liquid is reduced, and the pollution to the environment is reduced; and the synthesis reaction of the kanamycin A has the advantages of mild conditions, easiness in separation and purification of the product, and no special equipment requirements.
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Paragraph 0055-0060; 0066-0071; 0078-0083; 0090-0095; 0101
(2019/01/23)
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- Synthesis of ring II/III fragment of Kanamycin: A new minimum structural motif for aminoglycoside recognition
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A novel protocol has been established to prepare the kanamycin ring II/III fragment, which has been validated as a minimum structural motif for the development of new aminoglycosides on the basis of its bactericidal activity even against resistant strains. Furthermore, its ability to act as a AAC-(6′) and APH-(3′) binder, and as a poor substrate for the ravenous ANT-(4′), makes it an excellent candidate for the design of inhibitors of these aminoglycoside modifying enzymes.
- Zárate, Sandra G.,Bastida, Agatha,Santana, Andrés G.,Revuelta, Julia
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- COMPOUNDS AND METHODS FOR MODULATING RNA FUNCTION
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The present invention provides compounds, compositions thereof, and methods of using the same.
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Paragraph 00410; 00411
(2018/02/28)
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- COMPOUNDS AND METHODS OF TREATING RNA-MEDIATED DISEASES
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The present invention provides compounds, compositions thereof, and methods of using the same.
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Paragraph 00350; 00351
(2017/12/27)
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- The last step of kanamycin biosynthesis: Unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK
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Mystery solved: Using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism (see scheme) to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered. Copyright
- Sucipto, Hilda,Kudo, Fumitaka,Eguchi, Tadashi
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supporting information; experimental part
p. 3428 - 3431
(2012/06/30)
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- NEW AMINOGLYCOSIDE COMPOUNDS AND DERIVATIVES THEREOF
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New amino glycoside compounds or derivatives thereof having at least one sugar moiety and which comprise two or more cyclic structures capable of forming “charmed” structural features at physiological pH, and which have binding affinities for RNA and protein structures and may be used as therapeutic or screening or diagnostic agents and the like.
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Page/Page column 64-65
(2008/06/13)
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- Site-specific aminoglycoside derivatives and their use in immunodiagnostic assays
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A method of making a derivatized aminoglycoside includes reacting an aminoglycoside with at least 2 equivalents of a divalent metal ion in an aprotic solvent to complex two neighboring amino group and hydroxyl group pairs; reacting the non-complexed amino groups with a protecting reagent to provide protecting groups; removing the divalent metal ion to provide two unprotected amino groups; reacting one of the unprotected amino groups with a reactive substance containing an linker, a carrier, or a label; and removing the protecting groups. This method can be used to produce novel compounds and reagents.
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- Process for preparing amikacin
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Described is a process for preparing Amikacin wherein 1-N-(L(-)γ-benzyloxycarbonylamino-α-hyroxybutyryl)-3,6'-di-N-benzyloxycarbonyl-Kanamycin A suspended in a suitable solvent, is treated with an aqueous solution of formic acid in the presence of a catalyst; the reaction mixture is charged on a ion exchange resin column to yield the desired product. Among the side-products Kanamycin A is obtained which is per se useful.
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- Process for preparing amikacin
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Described is a process for preparing Amikacin wherein 1--N-(L(-)-γ-benzyloxycarbonylamino-α-hydroxybutyryl)-3,6?-di--N-benzyloxycarbonyl-Kanamycin A suspended in a suitable solvent, is treated with an aqueous solution of formic acid in the presence of a catalyst; the reaction mixture is charg-ed on a ion exchange resin column to yield the desired pro-duct. Among the side-products Kanamycin A is obtained which is per seuseful.
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- SYNTHESES OF METHYL 6-DEOXY-6-HYDROXYAMINO-α-D-GLUCOPYRANOSIDE, 6'-N-HYDROXYKANAMYCIN A, AND 6'-N-HYDROXYDIBEKACIN
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Methyl-6-deoxy-6-hydroxyamino-α-D-glucopyranoside has been prepared from methyl 6-amino-6-deoxy-α-D-glucopyranoside via oxidation with hydrogen peroxide in the presence of sodium tungstate , followed by reduction with sodium cyanoborohydride in an acidic medium.Acetylation of the Z isomer of 2 gave a nitrile derivative.The above oxidation-reduction procedure was applied to kanamycin A and dibekacin, starting from the corresponding 6'-amino-N-tosyl derivatives.On treatment with sodium in liquid ammonia, 6'-deamino-6'-hydroxyimino-1,3,3''-tri-N-tosylkanamycin A gave the corresponding N-detosyl derivative in good yield with the 6'-aldoxime group remaining intact, but, with 6'-deamino-6'-hydroxyimino-1,3,2',3''-tetra-N-tosyldibekacin, the N-detosyl derivative (17) was obtained only by a very short reaction period.The antibacterial activities of 17 and 6'-N-hydroxydibekacin were demonstrated.
- Tsuchiya, Tsutomu,Nakano, Masato,Torii, Takahiro,Suzuki, Yukiko,Umezawa Sumio
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p. 195 - 206
(2007/10/02)
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