- The fluorescent dye, synthesis method and use thereof (by machine translation)
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The present invention provides a category of cyanine dyes having the following general structural Formula I, wherein X is defined as C(CH3)2, O, S or Se; m is an integer from 1 to 18, R1 and R2 are each independently chosen from H, C1-18alkyl, OR7, C1-6alkyl-OR7 or halogen; R3 is pyrrolyl, imidazolyl, piperidinyl, piperazinyl, morpholinyl, guanidino, NHR5 or N(R6)2; R4 is C1-18alkyl, benzyl or (CH2)mR3; R5 is saturated and/or unsaturated, straight-chained and/or branched-chained C1-18alkyl, hydroxy alkyl, mercapto-alkyl, amino alkyl, acyl, phenyl, naphthyl or benzyl; R6 is a C2-18alkyl; R7 is H or a C1-18alkyl; Y? is an anion. The mentioned compounds can be applied to staining biological samples, to the fields of nucleic acids labeling, blood cell analysis, clinical diagnosis, immunological assays and etc.
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Paragraph 0078; 0079
(2016/10/09)
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- An effective minor groove binder as a red fluorescent marker for live-cell DNA imaging and quantification
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Seeing red: DEAB-TO-3 shows a large fluorescence enhancement upon binding to native DNA and a distinct selectivity for double-stranded DNA over total RNA. As a red fluorescent live-cell-permeant DNA minor groove binder, DEAB-TO-3 is promising for highly sensitive DNA detection in vitro and nucleus-specific imaging and DNA quantification in vivo (see picture). Copyright
- Peng, Xiaojun,Wu, Tong,Fan, Jiangli,Wang, Jingyun,Zhang, Si,Song, Fengling,Sun, Shiguo
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p. 4180 - 4183
(2011/07/07)
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