Development of a phage chemiluminescent enzyme immunoassay with high sensitivity for the determination of IMidaclothiz (cas 105843-36-5) in agricultural and environmental samples

Add time:07/19/2019    Source:sciencedirect.com

In this study, we isolated six phage-displayed peptides by biopanning phage-displayed peptide libraries on an immobilized anti-IMidaclothiz (cas 105843-36-5) monoclonal antibody. After analyzing the relative sensitivity of the individual phage-displayed peptides, we subsequently developed and optimized both a phage enzyme immunoassay (P-ELISA) and a phage chemiluminescent enzyme immunoassay (P-CLEIA) to improve the sensitivity and linear range of imidaclothiz assays. The P-CLEIA (50% inhibition concentration (IC50) of 0.86 ng mL− 1, linear range of 0.13–5.84 ng mL− 1) was more sensitive and had a wider linear range compared to the P-ELISA (IC50 of 1.45 ng mL− 1, linear range of 0.55–3.82 ng mL− 1). Besides, the sensitivities of the P-ELISA and P-CLEIA were increased by > 4-fold and 8-fold, respectively as compared to homologous immunoassays developed using the same monoclonal antibody. Neither method had significant cross-reactivity with the analogues of imidaclothiz except for imidacloprid. Recoveries of the P-ELISA and P-CLEIA for imidaclothiz in paddy water, soil, cabbage, rice, apple, pakchoi, pear and tomato samples were 72.3–101.3% and 73.9–102.6%, respectively. The P-ELISA and P-CLEIA detected imidaclothiz in the authentic samples, and showed good correlation with results obtained from high-performance liquid chromatography (HPLC).

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