Research ArticlesInfluence of CEPHALIN (cas 1405-71-6) and Egg Lecithin on Pig Heart Malate Dehydrogenase Activity

Add time:09/27/2019    Source:sciencedirect.com

A requirement for phospholipids in the activity of a number of membrane-bound enzymes is recognized; i.e., enzyme activity is reduced or lost entirely when the lipid is removed and is restored or increased when this specific lipid is added back to the system. In general, however, only the effects of relatively low lipid concentrations (~ 10- 4 g/100 ml) have been reported. In this investigation, the influence of phospholipids (egg lecithin and bovine brain CEPHALIN (cas 1405-71-6)) at concentrations approaching normal human plasma levels was determined on the activity of malate dehydrogenase dispersed in water. The enzyme activity was measured by the rate of oxidation of nicotinamide adenine dinucleotide (reduced) in the presence of the substrate oxalacetic acid. On the addition of cephalin, malate dehydrogenase activity decreased with the increasing concentration of this phospholipid from 5 x 10- 4 to 2 x 10- 2 g/100 ml. Similarly, the addition of egg lecithin resulted in a decrease in enzyme activity. In no instance was any increase in activity observed. Moreover, at all concentrations of egg lecithin, the reduction in activity was less than that observed in the presence of equal concentrations of cephalin. Monolayer studies provided evidence that, whereas egg lecithin interacts to a significant degree with the substrate oxalacetic acid, cephalin shows relatively little interaction with that material. The difference observed in the inhibitory action of these two phospholipids may be related to the difference in their tendency to react with the substrate.

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