Efficient biotransformation of d-fructose to d-mannose by a thermostable D-LYXOSE (cas 1114-34-7) isomerase from Thermosediminibacter oceani

Add time:08/14/2019    Source:sciencedirect.com

d-Mannose has prebiotic effect and potential medical application. Besides, it can be used as substrate to produce mannitol, a functional polyol widely used in food industry. As this result, it has attracted many researchers’ attention. In this work, a thermostable d-mannose-producing D-LYXOSE (cas 1114-34-7) isomerase (D-LI) was characterized from a hyperthermophile, Thermosediminibacter oceani. The recombinant D-LI could be remarkably activated by Mn2+. It displayed maximal activity in presence of 1 mM Mn2+ at pH 6.5 and 65 °C, and was determined to be highly thermostable at 80 °C. The half-life was calculated to be 5.64, 2.82, 0.77, and 0.2 h at 70, 75, 80, and 85 °C, respectively. The enzyme showed the optimum activity using d-lyxose as substrate and could also effectively catalyze the isomerization between d-fructose and d-mannose. Under optimum conditions, 101.6 g/L d-mannose was produced from 400 g/L d-fructose after reaction for 9 h, giving a conversion yield of 25.4%.

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