9004-07-3 Usage
Description
Chymotrypsin is a proteolytic enzyme that is primarily derived from the pancreas of cows. It is known for its ability to break down proteins into smaller peptides and amino acids, making it a valuable tool in various scientific and industrial applications.
Uses
Used in Anticancer Research:
Chymotrypsin is used as a proteasome inhibitor in anticancer research, helping to advance the understanding of cancer cell mechanisms and the development of potential treatments.
Used in Sperm Penetration Studies:
Chymotrypsin is used as a limited proteolysis agent to assess the effect of its action on sperm penetration, providing insights into fertility and reproductive health.
Used in Surface Functionalization:
Chymotrypsin is used as a functionalizing agent for surface-anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers, enabling the development of new materials and technologies.
Used in Protein Extraction Research:
Chymotrypsin from bovine pancreas is used as a protein extraction agent in studies involving Winsor-III microemulsion systems, contributing to the advancement of protein purification and analysis techniques.
Used in Fluorescent Probe Development:
Chymotrypsin is used as a target enzyme in the investigation of new specific fullerene-based fluorescent probes for trypsin, aiding in the development of innovative diagnostic tools and imaging agents.
Biochem/physiol Actions
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+.
Purification Methods
α-Chymotrypsin is crystallised twice from four-tenths saturated ammonium sulfate solution, then dissolved in 1mM HCl and dialysed against 1mM HCl at 2-4o. The solution is stored at 2o [Lang et al. J Am Chem Soc 80 4923 1958].
Check Digit Verification of cas no
The CAS Registry Mumber 9004-07-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,0 and 4 respectively; the second part has 2 digits, 0 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 9004-07:
(6*9)+(5*0)+(4*0)+(3*4)+(2*0)+(1*7)=73
73 % 10 = 3
So 9004-07-3 is a valid CAS Registry Number.
