9003-98-9 Usage
Description
Deoxyribonuclease, also known as DNase, is an enzyme that catalyzes the hydrolysis of DNA molecules. It is primarily found in the pancreas of mammals and plays a crucial role in the degradation and turnover of DNA in various biological processes.
Uses
Used in Molecular Biology:
Deoxyribonuclease I is used as a reagent for the removal of DNA from protein samples, ensuring the purity of proteins for further analysis and experiments.
Used in DNA Manipulation:
DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA, allowing for the tracking and visualization of DNA during various molecular biology techniques.
Used in Cell Biology:
The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture, aiding in the study of cellular processes and membrane dynamics.
Used in Histology and Tissue Preparation:
Deoxyribonuclease I has been used along with trypsin for the preparation of single cell suspensions from rat testes, facilitating the study of cell populations and their characteristics.
Used in Enzyme Purification:
Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase, ensuring the specificity and purity of enzymatic reactions.
Used in Enzyme Renaturation:
Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis, providing insights into protein folding and stability.
Used in RNase-free Applications:
Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples, preventing interference from RNA in DNA-related experiments.
Used in RNA Isolation and Purification:
Amplification grade DNase I has been used for the digestion of DNA during isolation and purification of RNA, ensuring the integrity of RNA samples for downstream applications such as cDNA synthesis using RNA reverse transcriptase.
Flammability and Explosibility
Notclassified
Biochem/physiol Actions
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Clinical Use
DNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I.Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy.
Check Digit Verification of cas no
The CAS Registry Mumber 9003-98-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,0 and 3 respectively; the second part has 2 digits, 9 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 9003-98:
(6*9)+(5*0)+(4*0)+(3*3)+(2*9)+(1*8)=89
89 % 10 = 9
So 9003-98-9 is a valid CAS Registry Number.
