9001-99-4 Usage
Description
Ribonuclease A (RNase A) is a pancreatic ribonuclease enzyme that cleaves single-stranded RNA. It is found in various sources, including bovine pancreas, and has been extensively studied for its structure, function, and potential applications in various fields.
Uses
Used in Molecular Biology:
Ribonuclease A is used as an enzyme for removing RNA from DNA plasmid preparations and protein samples. This is crucial for preventing contamination and ensuring accurate results in molecular biology experiments.
Used in RNase Protection Assays:
Ribonuclease A is utilized in RNase protection assays to remove unspecifically bound RNA and analyze RNA sequences. This helps in understanding gene expression and regulation.
Used in Cancer Research:
Ribonuclease A and its homologs have shown cytotoxic effects on cancer cells, making them potential candidates for cancer therapeutics. They have been studied for their potential to target and kill cancer cells while sparing healthy cells.
Used in Protein Structure and Absorbance Studies:
Ribonuclease A has been used as a model system in the study of protein structure, absorbance, and computer-aided drug design. Its well-characterized structure and function make it an ideal candidate for these studies.
Used in Chromatography Research:
Ribonuclease A from bovine pancreas has been used in studies to assess the mechanism of heavy metal ions on RNase activity and to investigate the performance of various chromatography techniques, such as particle-based and monolithic columns for cation exchange protein displacement chromatography.
Used in Human Genetics:
Human chromosome 14q11.2 shows the presence of all human ribonucleases, with eight of them being canonical ribonucleases (RNase 1-8) and the rest being noncanonical ribonucleases. Human RNase 1 is also known as HP-RNase or human pancreatic RNase, while Human RNase 2 is called EDN (eosinophils derived neurotoxin) and is isolated from eosinophils. Ribonuclease A plays a role in understanding the genetic basis of ribonuclease function and its implications in human health and disease.
Biochem/physiol Actions
Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
Purification Methods
Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins.
Check Digit Verification of cas no
The CAS Registry Mumber 9001-99-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,0 and 1 respectively; the second part has 2 digits, 9 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 9001-99:
(6*9)+(5*0)+(4*0)+(3*1)+(2*9)+(1*9)=84
84 % 10 = 4
So 9001-99-4 is a valid CAS Registry Number.