7423-31-6

Basic information

• Product Name: STAINS-ALL
• Synonyms: 1-Ethyl-2-((1E,3E)-3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methylprop-1-en-1-yl)napht;CARBOCYANIN DBTC;DBTC;3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACARBOCYANINE;3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACARBOCYANINE BROMIDE;3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACORBOCYANINE;3,3'-DIETHYL-9-METHYL-4,5,4',5'-NAPHTHOTHIACARBOCYANINE BROMIDE;1-ETHYL-2-[3-(1-ETHYLNAPHTHO[1,2-D]THIAZOLIN-2-YLIDENE)-2-METHYLPROPENYL]NAPHTHO[1,2-D]THIAZOLIUM BROMIDE
• CAS NO: 7423-31-6
• Molecular Formula: Br*C₃₀H₂₇N₂S₂
• Molecular Weight: 559.58
• EINECS: 231-047-7
• Product Categories: Naphthothiazole
• Mol File: 7423-31-6.mol
• Article Data: 0

Chemical Properties

• Melting Point: >200℃
• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: Dull dark purplish to dark gray/Dull PurplTo Powder
• Density: 1.3339 (estimate)
• Storage Temp.: 2-8°C
• Solubility: Soluble in water, ethanol, chloroform
• Water Solubility: Soluble in water.
• Sensitive: Light Sensitive
• BRN: 3865213
• CAS DataBase Reference: STAINS-ALL(CAS DataBase Reference)
• NIST Chemistry Reference: STAINS-ALL(7423-31-6)
• EPA Substance Registry System: STAINS-ALL(7423-31-6)

Safety Data

• Safety Statements: 24/25
• WGK Germany: 3
• F: 3-8-10
• TSCA: Yes
• Hazardous Substances Data: 7423-31-6(Hazardous Substances Data)

Usage

Description

STAINS-ALL is a versatile dye that is primarily used for the visualization and identification of proteins on polyacrylamide gels. It differentiates between highly acidic proteins, which stain blue, and less acidic proteins, which stain pink. Additionally, it can be used as a nucleic acid and protein stain, making it a valuable tool in various scientific applications.

Uses

Used in Biochemistry and Molecular Biology:
STAINS-ALL is used as a protein stain for the visualization and identification of proteins on polyacrylamide gels. It allows for the differentiation between highly acidic proteins, which appear blue, and less acidic proteins, which appear pink. This feature makes it an ideal dye for analyzing protein samples in research and diagnostic settings.
Used in Gel Electrophoresis:
In gel electrophoresis, STAINS-ALL is used as a staining agent to enhance the visibility of proteins within the gel matrix. The intensity of STAINS-ALL can be increased by the addition of silver nitrate, further improving the detection of proteins.
Used in Nucleic Acid Analysis:
STAINS-ALL is also used as a nucleic acid stain, making it a valuable tool for the detection and analysis of DNA and RNA in various experimental procedures. Its ability to bind to both proteins and nucleic acids broadens its applicability in molecular biology research.

InChI:InChI=1/C30H27N2S2/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28/h6-19H,4-5H2,1-3H3/q+1

Synthetic route

methyl dithioacetate (2168-84-5) + 1-ethyl-2-methylnaphtho[1,2-d]thiazolium p-toluenesulphonate (42952-29-4) → 1-(1-ethyl-1H-naphtho[1,2-d]thiazol-2-ylidene)-propane-2-thione (68162-26-5) + 1,3-bis-(1-ethyl-naphtho[1,2-d]thiazol-2-yl)-2-methyl trimethinium ; bromide (7423-31-6)

Conditions	Yield
With pyridine; triethylamine anschliessend Behandeln mit wss. KBr;

1-ethyl-2-methylnaphtho[1,2-d]thiazolium p-toluenesulphonate (42952-29-4) + Triethyl orthoacetate (78-39-7) → 1,3-bis-(1-ethyl-naphtho[1,2-d]thiazol-2-yl)-2-methyl trimethinium ; bromide (7423-31-6)

Conditions	Yield
With pyridine Behandeln des Reaktionsprodukts in Methanol mit wss. KBr;

Upstream product

• 2168-84-5 methyl dithioacetate 
• 42952-29-4 1-ethyl-2-methylnaphtho[1,2-d]thiazolium p-toluenesulphonate 
• 78-39-7 Triethyl orthoacetate