685898-44-6 Usage
Uses
Used in Cancer Therapy:
L-Phenylalanine, 4-[bis(2-chloroethyl)oxidoaMino]-, (Hydrochloride) (1:2) is used as a HIF-1α inhibitor for the treatment of solid tumors. It targets hypoxia-inducible factor-1 alpha (HIF-1α), a protein that plays a critical role in tumor growth and adaptation to low oxygen conditions. By inhibiting HIF-1α, this compound may help control tumor progression and improve the effectiveness of cancer therapies.
Used in Metabolic Research:
In the field of metabolic research, L-Phenylalanine, 4-[bis(2-chloroethyl)oxidoaMino]-, (Hydrochloride) (1:2) is used to study the effects of high-fat-diet (HFD)-induced HIFα activation in adipose tissue. It has been shown to suppress this activation in mice, suggesting its potential use in ameliorating adipose tissue dysfunction and contributing to a better understanding of obesity-related metabolic disorders.
Used in Pharmaceutical Development:
L-Phenylalanine, 4-[bis(2-chloroethyl)oxidoaMino]-, (Hydrochloride) (1:2) is utilized in the development of new pharmaceutical agents targeting various diseases. Its unique chemical structure allows for the design of novel drugs with specific mechanisms of action, potentially leading to more effective treatments for a range of conditions.
Used in Chemical Synthesis:
In the chemical synthesis industry, L-Phenylalanine, 4-[bis(2-chloroethyl)oxidoaMino]-, (Hydrochloride) (1:2) serves as a building block or intermediate in the synthesis of complex organic compounds. Its reactivity and functional groups make it a valuable component in the creation of new molecules with specific properties and applications.
Used in Biochemical Analysis:
L-Phenylalanine, 4-[bis(2-chloroethyl)oxidoaMino]-, (Hydrochloride) (1:2) is employed in biochemical analysis to study enzyme activity, protein interactions, and other biological processes. Its unique structure allows researchers to probe the mechanisms of various biochemical reactions and develop a deeper understanding of cellular functions.
Biological Activity
px-478 2hcl, also called px-478, is an experimental hif-1α inhibitor. it inhibited hif-1α protein at multiple levels in cancer cell lines under normoxia and hypoxia with an ic50 value of ~20-30 μm [1].hypoxia-inducible transcription factor hif-1α activates target genes involved in glycolysis, erythropoiesis and angiogenesis. hif-1α also regulates the expression of genes coding for apoptotic pathway, growth factors/receptors, cell cycle regulators, metastasis and invasiveness [1].under normoxic condition, the ic50 for px-478 to inhibit hif-1α in pc3 cells was 20-25 μmol/l, whereas the ic50 for px-478 to inhibit hif-1α in the du 145 cells was ~40-50 μmol/l. in both du 145 and pc3 cells, 1-hr hypoxia significantly upregulated hif-1α protein. but the pretreatment with px-478 for 20 hr under normoxia before 1-hr hypoxia attenuated the accumulation of hif-1α induced by the 1-hr hypoxia. in pc3 cells, pretreatment with 20 μmol/l px-478 before hypoxia inhibited hif-1α by 40% compared to treatment with hypoxia alone. in du 145 cells, pretreatment with 50 μmol/l px-478 before hypoxia inhibited hif-1α by 35% compared to treatment with hypoxia alone [1].in nu/nu mice with c6 reporter xenografts, treatment with px-478 at a dose of 30 mg/kg p.o. for 2 consecutive days prevented the transcriptional activity of hif-1 in central ischemic regions of c6 tumors [2].
references
[1]. palayoor st, mitchell jb, cerna d, et al. px-478, an inhibitor of hypoxia-inducible factor-1α, enhances radiosensitivity of prostate carcinoma cells. international journal of cancer, 2008, 123(10): 2430-2437.[2]. schwartz dl, powis g, thitai-kumar a, et al. the selective hypoxia inducible factor-1 inhibitor px-478 provides in vivo radiosensitization through tumor stromal effects. molecular cancer therapeutics, 2009, 8(4): 947-958.
Check Digit Verification of cas no
The CAS Registry Mumber 685898-44-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 6,8,5,8,9 and 8 respectively; the second part has 2 digits, 4 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 685898-44:
(8*6)+(7*8)+(6*5)+(5*8)+(4*9)+(3*8)+(2*4)+(1*4)=246
246 % 10 = 6
So 685898-44-6 is a valid CAS Registry Number.