5425-78-5 Usage
Description
S-Carboxyethylisothiuronium chloride, also known as CEI, is a chemical compound with the molecular formula C6H11ClN2S. It is a derivative of isothiourea and has a zwitterionic structure. S-Carboxyethylisothiuronium chloride is known for its various applications in different industries, particularly in molecular biology and biochemistry.
Uses
Used in Molecular Biology:
S-Carboxyethylisothiuronium chloride is used as a reagent for the detection and quantification of ATP (adenosine triphosphate) levels in biological samples. It acts by reacting with ATP to form a stable, light-emitting product, which can be measured using a luminometer. This application is crucial for understanding cellular energy metabolism and assessing the activity of enzymes involved in ATP production or consumption.
Used in Biochemical Assays:
S-Carboxyethylisothiuronium chloride is used as a component in various buffer solutions, such as loading buffers and in vitro transcription reaction mixtures. In these applications, it helps maintain the stability of the reaction components and ensures accurate results.
Used in DNA and RNA Manipulation:
In the field of molecular biology, S-Carboxyethylisothiuronium chloride is used as a component in ligation mixes. It aids in the process of joining DNA or RNA fragments together, which is essential for cloning, gene expression studies, and other genetic engineering techniques.
Biochem/physiol Actions
Adenosine triphosphate (ATP) is a central metabolite, which acts as an energy transfer molecule, a phosphate donor and a signaling molecule inside the cells. This extracellular signaling molecule also serves as a neurotransmitter in peripheral and central nervous systems. In the peripheral nervous system, ATP participates in chemical transmission.
Check Digit Verification of cas no
The CAS Registry Mumber 5425-78-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 5,4,2 and 5 respectively; the second part has 2 digits, 7 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 5425-78:
(6*5)+(5*4)+(4*2)+(3*5)+(2*7)+(1*8)=95
95 % 10 = 5
So 5425-78-5 is a valid CAS Registry Number.
InChI:InChI=1/C4H8N2O2S.ClH/c1-2(3(7)8)9-4(5)6;/h2H,1H3,(H3,5,6)(H,7,8);1H
5425-78-5Relevant articles and documents
Reactions of Thiourea with Chloro- and Bromoacetic Acids
Kavalek, Jaromir,Said-El-Bahaie,Sterba, Vojeslav
, p. 263 - 268 (2007/10/02)
The reaction of chloroacetic acid with thiourea represents an SN2 substitution of chlorine by sulphur atom and is about two orders of magnitude slower than that of bromoacetic acid.Chloroacetate ion reacts slower than chloroacetic acid by only about 30percent.The acid catalyzed splitting off of hydroxyl group from the formed tetrahedral intermediate is rate-limiting in the cyclization of the S-carboxylatomethyleneisothiouronium salt, formation of the intermediate being rate-limiting below pH 2.
