52278-77-0 Usage
Description
5-HYDROXY-2'-DEOXYCYTIDINE is a modified nucleoside that plays a significant role in various biological processes, particularly in the context of DNA repair and response to oxidative stress. It is formed when deoxycytidine, a nucleoside in DNA, undergoes oxidation due to exposure to reactive oxygen species.
Uses
Used in DNA Repair Studies:
5-HYDROXY-2'-DEOXYCYTIDINE is used as a substrate for uracil DNA N-glycosylase (UDG), an enzyme involved in the base excision repair (BER) pathway. This application helps researchers understand the role of UDG in repairing oxidatively damaged DNA and maintaining genomic integrity.
Used in Oxidative Stress Research:
5-HYDROXY-2'-DEOXYCYTIDINE is used to study the oxidation of DNA due to exposure to reactive oxygen species (ROS). This application is crucial for understanding the mechanisms of DNA damage and the subsequent cellular responses, which can lead to insights into the development of therapeutic strategies against oxidative stress-related diseases.
Used in Pharmaceutical Industry:
5-HYDROXY-2'-DEOXYCYTIDINE is used as a key intermediate in the synthesis of various nucleoside analogs, which are important for the development of antiviral and anticancer drugs. These analogs can be designed to target specific enzymes or pathways involved in disease progression, making them valuable tools in the pharmaceutical industry.
Used in Diagnostics:
5-HYDROXY-2'-DEOXYCYTIDINE can be used as a biomarker for oxidative stress and DNA damage in various diseases, including cancer and neurodegenerative disorders. Its detection and quantification can help in the development of diagnostic tools and personalized medicine approaches.
Used in Chemical Synthesis:
5-HYDROXY-2'-DEOXYCYTIDINE is used as a starting material for the synthesis of other modified nucleosides and analogs with potential applications in various fields, such as molecular biology, drug development, and materials science. Its unique chemical properties make it a valuable building block for the creation of novel compounds with specific functions and properties.
Check Digit Verification of cas no
The CAS Registry Mumber 52278-77-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,2,2,7 and 8 respectively; the second part has 2 digits, 7 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 52278-77:
(7*5)+(6*2)+(5*2)+(4*7)+(3*8)+(2*7)+(1*7)=130
130 % 10 = 0
So 52278-77-0 is a valid CAS Registry Number.
52278-77-0Relevant articles and documents
Reaction of 2′-deoxycytidine with peroxynitrite in the presence of ammonium bromide
Suzuki, Toshinori,Ida, Kazuya,Uchibe, Shinya,Inukai, Michiyo
, p. 5164 - 5170 (2008/12/20)
Peroxynitrite, a reactive nitrogen species generated from nitric oxide and superoxide anion radical, is an endogenous potential risk factor for human cancer. When 2′-deoxycytidine was incubated with peroxynitrite at neutral pH and 37 °C, the reaction was greatly enhanced by the addition of ammonium bromide. Both ammonium ion and bromide ion were required to exert the enhancing effect. In addition to ammonium ion, methylamine and dimethylamine exerted the enhancing effect in the presence of bromide ion. Two major products were identified as 5-hydroxy-2′-deoxycytidine and 5-bromo-2′-deoxycytidine. Hypochlorite solution and bromine water reacted with 2′-deoxycytidine generating 5-hydroxy-2′-deoxycytidine and 5-bromo-2′-deoxycytidine in the presence of ammonium bromide with the yields similar to those of the reaction of peroxynitrite with ammonium bromide. Fenton reaction of 2′-deoxycytidine was suppressed by the addition of ammonium bromide. Nitrogen dioxide gas did not react with 2′-deoxycytidine in the presence or the absence of ammonium bromide. These results suggest that in the presence of ammonium ion or amines, bromide ion interacts with peroxynitrous acid, which is a protonated form of peroxynitrite, but not with hydroxyl radical or nitrogen dioxide generated by homolysis of peroxynitrous acid, to form hypobromous acid. In the presence of ammonium ion or amines, bromide ion may play a role in enhancing the genotoxic effects of peroxynitrite in humans.