49705-99-9Relevant articles and documents
Synthesis method of aztreonam main ring synthesis intermediate N-carbobenzoxy-L-threonine amide
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Paragraph 0018-0024, (2020/05/01)
The invention provides a synthesis method of aztreonam main ring synthesis intermediate N-carbobenzoxy-L-threonine amide, which comprises the following steps: 1) adding an L-threonine solid and methanol into a reaction kettle, stirring, cooling, dropwisely adding thionyl chloride, carrying out heating reflux reaction, and drying a solvent by distillation; (2) adding methanol into a reactant obtained in the step (1) for dissolving, heating and pressurizing, introducing liquid ammonia, keeping the temperature and the pressure, evaporating the liquid ammonia after the reaction is finished, and evaporating the solvent to dryness; and 3) adding water into a reaction product obtained in the step 2) for dissolving, dropwise adding benzyl chloroformate, dropwise adding an acid-binding agent solution to control the pH value of the reaction system to obtain intermediate N-carbobenzoxy-L-threonine amide, and carrying out filter pressing and drying to obtain a solid.
HEMIAMINAL-TAG FOR PROTEIN LABELING AND PURIFICATION
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Page/Page column 22; 24, (2018/06/30)
The invention pertains to the synthesis, isolation, and characterization of hemiaminal for selective labeling of peptides, proteins, antibodies, and organic fragments with -C(=0) CH2NH2 and derivatives with -CH2NH2 group over -C(=0) CHRNH2 group (where R≠H). The invention also pertains to the method of single-site immobilization of proteins through N-terminus Gly on solid phase. The invention includes late-stage tagging of N-terminus Gly with an affinity tag, 19F NMR probe, and a fluorophore and a method for metal-free protein purification and isolation of analytically pure proteins.
PROCESSES FOR SYNTHESIS OF DIPYRROLIDINE PEPTIDE COMPOUNDS
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Paragraph 00103-00107, (2017/12/27)
Disclosed is a new process for preparing dipyrrolidine peptide compounds such as, for example, GLYX-13. Advantageously, the process may be industrially scalable and cost-effective and use less toxic reagents and/or solvents. Further, the process may be used to prepare peptide compounds having improved purity.