4419-94-7 Usage
Description
P-NITROPHENYL BETA-D-LACTOPYRANOSIDE, also known as 4-Nitrophenyl β-D-Lactopyranoside, is a synthetic compound commonly used in biochemical research and enzymatic assays. It is a chromogenic substrate, meaning it changes color upon enzymatic cleavage, which allows for the easy detection and quantification of enzymatic activity.
Uses
Used in Biochemical Research:
P-NITROPHENYL BETA-D-LACTOPYRANOSIDE is used as a substrate for determining β-lactosidase activity. P-NITROPHENYL BETA-D-LACTOPYRANOSIDE serves as an indicator of enzymatic activity, as the release of the 4-nitrophenyl group upon cleavage results in a color change that can be measured spectrophotometrically. This property makes it a valuable tool for studying enzyme kinetics, inhibitor screening, and enzyme characterization.
Used in Enzyme Assays:
P-NITROPHENYL BETA-D-LACTOPYRANOSIDE is used as a substrate for cellobiohydrolase, an enzyme involved in the breakdown of cellulose. The color change upon enzymatic cleavage allows researchers to monitor the progress of the enzymatic reaction and assess the efficiency of the enzyme in breaking down cellulose.
Used in Determining Substrate Specificity:
P-NITROPHENYL BETA-D-LACTOPYRANOSIDE is used to determine the substrate specificity for β-glucosidase from the yeast Debaryomyces hansenii. By using this compound as a substrate, researchers can gain insights into the enzyme's preferences for specific substrates and its potential applications in various industrial processes, such as biofuel production or the synthesis of specific glycosides.
Check Digit Verification of cas no
The CAS Registry Mumber 4419-94-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 4,4,1 and 9 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 4419-94:
(6*4)+(5*4)+(4*1)+(3*9)+(2*9)+(1*4)=97
97 % 10 = 7
So 4419-94-7 is a valid CAS Registry Number.
InChI:InChI=1/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9?,10?,11-,12-,13+,14-,15-,16+,17+,18-/m0/s1
4419-94-7Relevant articles and documents
SYNTHETIC CATALYSTS FOR CARBOHYDRATE PROCESSING
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, (2022/01/24)
The disclosure relates to molecularly-imprinted cross-linked micelles that can selectively hydrolyze carbohydrates.
BEVERAGE DISPENSING EQUIPMENT
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Paragraph 00154; 00156, (2016/12/01)
Apparatus and methods for reducing biofouling in drink such as beer dispensing equipment are described.
Environmentally benign glycosylation of aryl pyranosides and aryl/alkyl furanosides demonstrating the versatility of thermostable CGTase from Thermoanaerobacterium sp.
Pennec, Alize,Legentil, Laurent,Herrera-Estrella, Luis,Ferrieres, Vincent,Chauvin, Anne-Laure,Nugier-Chauvin, Caroline
supporting information, p. 3803 - 3809 (2014/08/05)
An extensive study on the specificity of transglycosylation and disproportionation of Thermoanaerobacterium sp. cyclodextrin glucosyltransferases against aryl glucopyranosides or furanosides is reported. While a mixture of maltoside and isomaltoside was obtained respectively using p-nitrophenyl glucopyranoside as an acceptor, only one regioisomer, namely p-nitrophenyl α-d-Glcp-(1,3)-α-l-Araf, was isolated using p-nitrophenyl arabinofuranoside as an acceptor. Interestingly, similar outcomes were found when using p-nitrophenyl galactofuranoside. Furthermore, activation by microwave irradiation resulted in faster reaction times and higher yields and led to glucosidic oligosaccharides with up to 70% conversion. The influence of the anomeric and C-4 configurations of the glycosidic acceptors on the transglycosylation, previously stated for the CGTase family, was not observed and unconventional substrate specificity towards alkyl furanosides was highlighted. This journal is the Partner Organisations 2014.
