425427-87-8

Basic information

• Product Name: 6-CHLORO-3-INDOXYL-BETA-D-CELLOBIOSIDE
• Synonyms: 6-CHLORO-3-INDOXYL-BETA-D-CELLOBIOSIDE;6-CHLORO-3-INDOLYL BETA-D-CELLOBIOPYRANOSIDE;ROSE(TM)-CELLOBIOSIDE;SALMON(TM)-BETA-D-CELLOBIOSIDE;6-Chloro-3-indolylb-D-cellobioside;Salmon-cellobioside;6-Chloro-3-indolyl beta-D-cellobioside;6-Chloro-3-indolyl β-D-cellobioside
• CAS NO: 425427-87-8
• Molecular Formula: C20H26ClNO11
• Molecular Weight: 491.87
• EINECS: 1533716-785-6
• Product Categories: substrate
• Mol File: 425427-87-8.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 834.2 °C at 760 mmHg
• Flash Point: 458.3 °C
• Appearance: /
• Density: 1.72g/cm³
• Vapor Pressure: 1.64E-29mmHg at 25°C
• Refractive Index: 1.716
• PKA: 12.71±0.70(Predicted)
• CAS DataBase Reference: 6-CHLORO-3-INDOXYL-BETA-D-CELLOBIOSIDE(CAS DataBase Reference)
• NIST Chemistry Reference: 6-CHLORO-3-INDOXYL-BETA-D-CELLOBIOSIDE(425427-87-8)
• EPA Substance Registry System: 6-CHLORO-3-INDOXYL-BETA-D-CELLOBIOSIDE(425427-87-8)

Safety Data

• Hazardous Substances Data: 425427-87-8(Hazardous Substances Data)

Usage

Description

6-Chloro-3-Indoxyl-beta-D-cellobioside is a synthetic chromogenic substrate that is specifically designed to interact with the enzyme β-D-Cellobiosidase. It is characterized by its ability to produce a distinct salmon-colored precipitate upon enzymatic action, making it a useful tool for detecting and studying the activity of this particular enzyme.

Uses

Used in Enzyme Assays:
6-Chloro-3-Indoxyl-beta-D-cellobioside is used as a chromogenic substrate for enzyme assays, specifically for the detection and quantification of β-D-Cellobiosidase activity. The formation of a salmon-colored precipitate upon enzymatic action allows for the visualization and measurement of enzyme activity, which is crucial in various research and diagnostic applications.
Used in Research and Diagnostics:
In the field of research and diagnostics, 6-Chloro-3-Indoxyl-beta-D-cellobioside is employed as a valuable tool for studying the function and role of β-D-Cellobiosidase in various biological processes. The distinct color change produced by this substrate enables researchers to monitor enzyme activity, assess the effectiveness of potential enzyme inhibitors, and investigate the underlying mechanisms of enzyme-catalyzed reactions.
Used in Pharmaceutical Development:
6-Chloro-3-Indoxyl-beta-D-cellobioside may also be utilized in the development of new pharmaceuticals targeting β-D-Cellobiosidase or related enzymes. By understanding the enzyme's activity and its role in disease processes, researchers can design and test potential drug candidates that modulate enzyme function, potentially leading to novel therapeutic strategies for various conditions.
Used in Analytical Chemistry:
In analytical chemistry, 6-Chloro-3-Indoxyl-beta-D-cellobioside can be employed as a colorimetric indicator for the detection and quantification of β-D-Cellobiosidase in complex biological samples. The substrate's specificity and the distinct color change it produces make it a reliable and sensitive tool for the analysis of enzyme activity in various experimental setups.
Used in Quality Control:
6-Chloro-3-Indoxyl-beta-D-cellobioside can be utilized in the quality control of enzyme preparations, ensuring that the enzyme's activity is within the desired range. By monitoring the formation of the salmon-colored precipitate, manufacturers can verify the potency and consistency of their enzyme products, ensuring that they meet the required standards for research, diagnostic, and pharmaceutical applications.

InChI:InChI=1/C20H26ClNO11/c21-7-1-2-8-9(3-7)22-4-10(8)30-19-17(29)15(27)18(12(6-24)32-19)33-20-16(28)14(26)13(25)11(5-23)31-20/h1-4,11-20,22-29H,5-6H2/t11-,12-,13+,14+,15-,16-,17-,18+,19-,20+/m0/s1