39445-21-1 Usage
Description
Elastase is a single polypeptide chain enzyme consisting of 240 amino acid residues and containing four disulfide bridges. It has a molecular mass of approximately 25.9 kDa and is synthesized as an inactive zymogen called proelastase, which is then converted to its active form through limited proteolysis at the N-terminal by trypsin.
Uses
Used in Mass Spectrometry:
Elastase is used in combination with other proteases for protein analysis by mass spectrometry. It aids in breaking down proteins into smaller fragments, which can be more easily analyzed and identified.
Used in Tissue Dissociation:
Elastase is utilized in tissue dissociation due to its ability to break down elastin, which is found in high concentrations in the elastic fibers of connective tissues. It is often used alongside other enzymes such as collagenase, trypsin, and chymotrypsin to dissociate tissues with extensive intercellular fiber networks.
Used in Membrane Protein Solubilization:
Elastase is employed in the solubilization of membrane proteins, which is a crucial step in various biochemical and biophysical studies.
Used in Protein Sequence Studies:
This enzyme is also used in protein sequence studies, where it helps in the identification and characterization of protein sequences.
Used in Bone/Calcium Metabolism Research:
Elastase from porcine pancreas has been used in studies to identify novel factors regulating bone/calcium metabolism.
Used in Acute Hemorrhagic Pancreatitis Research:
Elastase from porcine pancreas has been utilized in research to investigate the effects of specific synthetic inhibitors, such as ONO-5046, on the course of acute hemorrhagic pancreatitis in dogs.
Used in Cell Culture and Protease Assays:
Elastase from porcine pancreas has been used to treat vero cells, study its effects on syncytium formation, serve as a positive control in protease assays, and as a component in RPMI 1640 to isolate human aortic smooth muscle cells (HASMCs) from aortic tissue.
Biochem/physiol Actions
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
Check Digit Verification of cas no
The CAS Registry Mumber 39445-21-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,9,4,4 and 5 respectively; the second part has 2 digits, 2 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 39445-21:
(7*3)+(6*9)+(5*4)+(4*4)+(3*5)+(2*2)+(1*1)=131
131 % 10 = 1
So 39445-21-1 is a valid CAS Registry Number.
