38183-12-9 Usage
Description
Fluorescamine is a non-fluorescent reagent that readily reacts with primary amines in amino acids and peptides under mild conditions to form stable, highly fluorescent compounds. It is a white powder and is known for its low background due to hydrolysis.
Uses
Used in Analytical Chemistry:
Fluorescamine is used as a reagent for the fluorescent detection of amino acids, peptides, and proteins. It plays an important role in the detection and quantitation of these biomolecules, providing a sensitive and reliable method for their analysis.
Used in Enzyme Activity Measurement:
Fluorescamine is used as a substrate for measuring protease activity, allowing researchers to study the function and properties of these enzymes.
Used in Pharmaceutical Analysis:
Fluorescamine finds application in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by high-performance liquid chromatography (HPLC), ensuring the quality and safety of pharmaceutical products.
Used in Clinical Chemistry:
Fluorescamine is utilized in the determination of lisinopril in human plasma and urine, aiding in the assessment of drug levels for therapeutic monitoring and pharmacokinetic studies.
Synthesis Reference(s)
Journal of the American Chemical Society, 94, p. 5927, 1972 DOI: 10.1021/ja00771a084
Purification Methods
Fluram is a non-fluorescent reagent that reacts with primary amines to form highly fluorescent compounds. Purify it by dissolving (~1g) in Et2O/*C6H6 (1:1, 180 mL), washing with 1% aqueous NaHCO3 (50mL), drying (Na2SO4), and evaporating in a vacuum. Dissolve the residue in warm CH2Cl2 (5mL), dilute with Et2O (12mL) and refrigerate. Collect the solid and dry it in a vacuum. IR (CHCl3): 1810, 1745, 1722, 1625 and 1600 cm-1, and 1HNMR (CDCl3): 8.71 (s, -OHC=). [Weigele et max al. J Am Chem Soc 94 5927 1972, Weigele et al. J Org Chem 41 388 1976, Lai Methods Enzymol 47 236 1977.] Forskolin (Colforsin, Coleonol, 5-[acetyloxy]-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7, 10a-penta-methyl-[3R -{3
Check Digit Verification of cas no
The CAS Registry Mumber 38183-12-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,8,1,8 and 3 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 38183-12:
(7*3)+(6*8)+(5*1)+(4*8)+(3*3)+(2*1)+(1*2)=119
119 % 10 = 9
So 38183-12-9 is a valid CAS Registry Number.
InChI:InChI=1/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H/t17-/m1/s1
38183-12-9Relevant articles and documents
Technological method for preparing fluorescent amine (by machine translation)
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Paragraph 0043; 0049-0057; 0059; 0065-0073, (2020/05/30)
The method, comprises the following steps: steps: reaction of an indene triketone compound as a raw material, with a phenylacetaldehyde compound in a proper amount of catalyst to obtain the diepoxide, and; reaction of the starting: with a proper amount of an oxidizing agent to obtain the diepoxide compound, has a short synthesis route, process and a simple, reaction. yield. (by machine translation)
Enkephalin analogues
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, (2008/06/13)
Compounds corresponding in structure to enkephalin or polypeptide analogues thereof, wherein one or more peptide links of the enkephalin or analogue is represented by a group or groups the same or different selected from dimethylene, methylene-imino and keto-methylene groups.
