37278-89-0

Basic information

• Product Name: EC 3.2.1.8
• Synonyms: XYLANASE;XYLANASE, RECOMBINANT;1,4-BETA-D-XYLANXYLANOHYDROLASE;EC 3.2.1.8;1,4-β-D-Xylanxylanohydrolase;endo-1,4-β-Xylanase ;Xylanase from Trichoderma viride;xylanase from aureobasidium pullulans
• CAS NO: 37278-89-0
• Molecular Formula: MW:
• Molecular Weight: 0
• EINECS: 253-439-7
• Product Categories: Enzymes
• Mol File: 37278-89-0.mol
• Article Data: 0

Chemical Properties

• Appearance: slightly yellow/powder
• Storage Temp.: 2-8°C
• CAS DataBase Reference: EC 3.2.1.8(CAS DataBase Reference)
• NIST Chemistry Reference: EC 3.2.1.8(37278-89-0)
• EPA Substance Registry System: EC 3.2.1.8(37278-89-0)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 37278-89-0(Hazardous Substances Data)

Usage

Description

EC 3.2.1.8, also known as Xylanase, is a type of enzyme that belongs to the glycoside hydrolase family. It catalyzes the hydrolysis of β-1,4-D-xylan, a major component of hemicellulose in plant cell walls, into xylose and other oligosaccharides. Xylanase has a wide range of applications in various industries due to its ability to break down xylan, making it a valuable tool in waste prevention and resource utilization.

Uses

Used in the Pulp and Paper Industry:
EC 3.2.1.8 is used as a biocatalyst for the hydrolytic degradation of xylan, which helps in the isolation of hemicellulose and production of oligosaccharides. This process aids in the efficient utilization of lignocellulosic biomass and contributes to waste prevention.
Used in the Food and Beverage Industry:
In the aqueous enzymatic extraction of corn oil from corn germ, EC 3.2.1.8 is used as a comparative tool for evaluating the efficiency of commercial enzymes. Its ability to break down xylan in corn germ enhances the extraction process and improves the quality of the extracted oil.
Used in the Viscosimetric Assay:
Xylanase is employed as a key component in viscosimetric assays, where it helps in the measurement of xylan viscosity. This application is crucial for understanding the properties of xylan and its potential uses in various industries.
Used in Cellulosic Ethanol Research:
EC 3.2.1.8 is used as an enhancer for waste prevention in cellulosic ethanol research. By breaking down xylan into simpler sugars, xylanase facilitates the production of ethanol from lignocellulosic biomass, making it a sustainable and environmentally friendly alternative to traditional fossil fuels.
Used in the Hydrolytic Degradation of Xylan:
In the hydrolytic degradation of xylan, EC 3.2.1.8 is used as a catalyst to break down the complex xylan structure into simpler components. This process is essential for the efficient utilization of lignocellulosic biomass and contributes to the development of sustainable bio-based products and energy sources.

Biochem/physiol Actions

Primary activity is an acid-neutral endo-1,4-β-D-xylanase, additional activities include β-glucanase, cellulase, pectinase, mannanase, xyloglucanase, laminarase, β-glucosidase, β-xylosidase, α-L-arabinofuranosidase, amylase, and protease.

Purification Methods

This xylanase is purified by anion-exchange chromatography on an Accell QMA column and finally by HPLC using a ProteinPak DEAE 5PW anion-exchange column. Solutions are stored frozen at -70o. [Morosoli et al. Biochem J 239 587 1986, Wong et al. Microbiol Rev 52 305 1988.] Carotenoids are polyene pigments that are mostly naturally occurring in bacteria, plants and animals. They have been isolated from the natural sources and obtained first by extraction with solvents and then purified by column chromatography through Al2O3 of various grades, Ca(OH)2 alone or with CaCO3,,,MgO or Silica Gel and eluted with solvents of various polarities. The progress of separation can be followed visually because the bands of most carotenoids are of various colours. The bands can be collected by elution, or the column can be extruded and the bands cut out and extracted with a polar solvent, e.g. MeOH. This chromatography can be repeated with the separate bands, and finally the carotenoids are recrystallised to analytical purity. The purity can be checked by TLC on Silica Gel or Al2O3 plates or paper chromatography and eluted in two dimensions. Gas-liquid or HPLC has been used for preparative work as well as for checking the purity and identifying them using internal standards such as tocopherol acetate (vitamin E acetate) and retinyl acetate. Carotenoids are generally light sensitive, easily oxidised by air and are affected by traces of acid, e.g. in solvents. These cause the polyenes to bleach or polymerize. The necessary precautions are therefore required to minimize these effects during isolation, purification and storage. They are identified by their UV-VIS spectra, and their molar extinction coefficients at specific wavelengths ( max) have been used for characterisation and for quantitation. More recently ORD, CD, NMR, IR and mass spectroscopy have been used extensively. Bibliography Carotenoids: Karrer & Jucker, Elsevier, 1950. The Comparative Biochemistry of the Carotenoids: Goodwin, Chapman & Hall Ltd, 1952.