37189-34-7

Basic information

• Product Name: BROMELAIN FROM PINEAPPLE STEM 2 U/MG
• Synonyms: BROMELAIN FROM PINEAPPLE STEM 2 U/MG;Bromelain from pineapple stem;Bromelain pineapple stem;Bromelain, stem;BROMELAIN FROM PINEAPPLE STEMCHROMATOGRA PHICALLY P;ANANAS COMOSUS;Einecs 253-387-5;Bromelain from pineapple stem min. 0.4 DMC-U/mg
• CAS NO: 37189-34-7
• Molecular Formula: n.a.
• Molecular Weight: 0
• EINECS: 253-387-5
• Product Categories: BromelainNutrition Research;ProteaseApplication Index;3.4.x.x Peptidases;3.x.x.x Hydrolases;Biochemicals Found in Plants;Proteases&Protein Sequencing;ProteasesEnzymes, Inhibitors, and Substrates;Proteolytic Enzymes and Substrates;Proteolytic EnzymesEnzyme Class Index;Selective Proteolytic Enzymes;BromelainEnzymes, Inhibitors, and Substrates
• Mol File: 37189-34-7.mol
• Article Data: 0

Chemical Properties

• Appearance: yellow-brown/powder
• Storage Temp.: −20°C
• Solubility: H2O: soluble1.0mg/mL, clear, colorless
• CAS DataBase Reference: BROMELAIN FROM PINEAPPLE STEM 2 U/MG(CAS DataBase Reference)
• NIST Chemistry Reference: BROMELAIN FROM PINEAPPLE STEM 2 U/MG(37189-34-7)
• EPA Substance Registry System: BROMELAIN FROM PINEAPPLE STEM 2 U/MG(37189-34-7)

Safety Data

• Hazard Codes: Xn,Xi
• Statements: 36/37/38-42
• Safety Statements: 22-24-26-36/37-36
• WGK Germany: 1
• F: 10-21
• Hazardous Substances Data: 37189-34-7(Hazardous Substances Data)

Usage

Description

BROMELAIN FROM PINEAPPLE STEM 2 U/MG is a yellow-brown powder derived from the stem of the pineapple plant. It is a proteolytic enzyme with a serine protease activity, which means it breaks down proteins into smaller peptides and amino acids. This enzyme has various applications in different industries due to its unique properties.

Uses

Used in Pharmaceutical Industry:
BROMELAIN FROM PINEAPPLE STEM 2 U/MG is used as an anti-inflammatory agent for its ability to inhibit the biosynthesis of proinflammatory prostaglandins. This property makes it a valuable component in the development of medications aimed at reducing inflammation and pain.
Used in Medical Applications:
In the medical field, BROMELAIN FROM PINEAPPLE STEM 2 U/MG is used as a clotting efficiency reducer. Its ability to reduce clotting efficiency can be beneficial in preventing blood clot formation, which is crucial in certain medical conditions and post-surgical care.
Used in Food Industry:
BROMELAIN FROM PINEAPPLE STEM 2 U/MG is used as an enzyme in the food industry for its ability to make enzymatic hydrolysates of honeybee-collected pollen. This application helps in the production of protein-rich food products and enhances the nutritional value of the final product.

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Purification Methods

This protease has been purified via the acetone powder, G-75 Sephadex gel filtration and Bio-Rex 70 ion-exchange chromatography, and has  1cm 20.1 at 280nm. The protease from pineapple hydrolyses benzoyl glycine ethyl ester with a Km (app) of 210mM and kcat of 0.36 sec-1 . [Murachi Methods Enzymol 19 273 1970, Balls et al. Ind Eng Chem 33 950 1941.]