3012-71-3

Basic information

• Product Name: 6-FITC
• Synonyms: 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-4-ISOTHIOCYANATO-BENZOIC ACID;6-FITC;6-ISOTHIOCYANATO-FLUORESCEIN;FITC ISOMER II;FITC 'ISOMER II';FITC;FLUORESCEIN 6-ISOTHIOCYANATE, ISOMER 2;FLUORESCEIN ISOTHIOCYANATE (ISOMER II)
• CAS NO: 3012-71-3
• Molecular Formula: C₂₁H₁₁NO₅S
• Molecular Weight: 389.38
• EINECS: 248-207-7
• Product Categories: Fluorescent Labels and Indicators;Fluorescent Labels & Indicators
• Mol File: 3012-71-3.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 719.1°C at 760 mmHg
• Flash Point: 388.7°C
• Appearance: /
• Density: 1.54g/cm³
• Vapor Pressure: 2.28E-21mmHg at 25°C
• Refractive Index: 1.753
• Storage Temp.: 2-8°C
• CAS DataBase Reference: 6-FITC(CAS DataBase Reference)
• NIST Chemistry Reference: 6-FITC(3012-71-3)
• EPA Substance Registry System: 6-FITC(3012-71-3)

Safety Data

• Hazard Codes: Xi
• Statements: 36-42/43
• Safety Statements: 26-45-36/37-22
• WGK Germany: 3
• Hazardous Substances Data: 3012-71-3(Hazardous Substances Data)

Usage

Description

6-FITC, also known as 6-carboxyfluorescein, is a fluorescent labeling reagent for proteins. It is a derivative of fluorescein, a synthetic organic compound with bright green-yellow fluorescence. The addition of the carboxyl group allows for covalent binding to proteins, making it a versatile tool in various applications.

Uses

Used in Biomedical Research:
6-FITC is used as a fluorescent probe for labeling proteins, enabling researchers to track and visualize their behavior in biological systems. This is particularly useful in studying protein-protein interactions, cellular localization, and dynamics.
Used in Diagnostic Applications:
In the fluorescent antibody technique, 6-FITC is used as a rapid identification tool for detecting and differentiating pathogens. The specific binding of antibodies labeled with 6-FITC to target antigens allows for quick and accurate diagnosis of diseases.
Used in Immunoassays:
6-FITC is employed as a label in various immunoassay formats, such as enzyme-linked immunosorbent assays (ELISA) and immunofluorescence assays. Its fluorescent properties facilitate the sensitive detection of antigen-antibody interactions.
Used in Drug Delivery and Imaging:
6-FITC can be used to label drug molecules or nanoparticles for tracking their biodistribution and pharmacokinetics. This helps in optimizing drug delivery systems and understanding their behavior in the body.
Used in Cell Biology:
In cell biology, 6-FITC is used for labeling cellular components, such as membranes, organelles, or specific proteins, to study their function and localization within cells.
Used in Environmental Research:
6-FITC can be employed to label environmental pollutants or contaminants, allowing for their detection and monitoring in various ecosystems.

InChI:InChI=1/C21H11NO5S/c23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h1-9,23-24H