17685-05-1 Usage
Description
Phenyl-β-D-glucuronide, also known as a metabolite of Phenol, is a glucuronide conjugate of phenol. It is an off-white solid that plays a significant role in various applications due to its chemical properties. It is primarily used as a reference standard in chromatographic and mass spectrophotometry studies for quantifying glucuroconjugate metabolites in plasma and urine samples.
Uses
1. Used in Chromatographic and Mass Spectrometry Studies:
Phenyl-β-D-glucuronide is used as a reference standard for the quantification of glucuroconjugate metabolites in plasma and urine samples. This application is crucial for accurate analysis and understanding of the metabolic processes involving phenol and its conjugates.
2. Used in Biuret Reagent Testing:
Phenyl-β-D-glucuronide serves as a nonpeptide chromogen for testing biuret reagent. This use is essential in the field of biochemistry, where biuret reagent is employed to detect the presence of proteins based on the formation of a purple complex.
3. Used in Urine Sample Analysis:
Phenyl-β-D-glucuronide is utilized as a reference standard for phenol analysis in urine samples. This application aids in the accurate measurement of phenol and its metabolites in urine, which can be vital for assessing an individual's health and understanding their metabolic processes.
Check Digit Verification of cas no
The CAS Registry Mumber 17685-05-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,7,6,8 and 5 respectively; the second part has 2 digits, 0 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 17685-05:
(7*1)+(6*7)+(5*6)+(4*8)+(3*5)+(2*0)+(1*5)=131
131 % 10 = 1
So 17685-05-1 is a valid CAS Registry Number.
17685-05-1Relevant articles and documents
Experimental and kinetic studies of the Escherichia coli glucuronylsynthase: An engineered enzyme for the synthesis of glucuronide conjugates
Wilkinson, Shane M.,Watson, Morgan A.,Willis, Anthony C.,McLeod, Malcolm D.
supporting information; experimental part, p. 1992 - 2000 (2011/06/19)
The detection and study of glucuronide metabolites is essential in many fields including pharmaceutical development, sports drug testing, and the detection of agricultural residues. Therefore, the development of improved methods for the synthesis of glucuronide conjugates is an important aim. The glycosynthase derived from E. coli β-glucuronidase provides an efficient, scalable, single-step synthesis of β-glucuronides under mild conditions. In this article we report on experimental and kinetic studies of the E. coli glucuronylsynthase, including the influence of acceptor substrate, pH, temperature, cosolvents, and detergents, leading to optimized conditions for glucuronide synthesis. Enzyme kinetics also reveals that both substrate and product inhibition may occur in glucuronylsynthase reactions but that these effects can be ameliorated through the judicious choice of acceptor and donor substrate concentrations. An investigation of temporary polar substituents was conducted leading to improved aqueous solubility of hydrophobic steroidal acceptors. In this way the synthesis of the steroidal metabolite dehydroepiandrosterone 3-β-d-glucuronide was achieved in three steps and 86% overall yield from dehydroepiandrosterone.
