144110-42-9 Usage
General Description
5-Bromo-6-chloro-3-indolyl-β-D-glucuronide, also known as X-Gluc, is a chemical compound commonly used in molecular biology research as a substrate for β-glucuronidase, an enzyme produced by certain bacteria and plants. X-Gluc is often used in the detection and visualization of β-glucuronidase activity in cellular and tissue samples, as it can be hydrolyzed by the enzyme to produce a blue precipitate. 5-Bromo-6-chloro-3-indolylb-D-glucuronide is particularly useful in the study of gene expression and promoter activity in transgenic plants and bacteria, as well as in the detection of β-glucuronidase reporter gene systems in molecular biology experiments. Additionally, X-Gluc can also be used in histochemical staining procedures to localize β-glucuronidase expression within plant and bacterial tissues.
Check Digit Verification of cas no
The CAS Registry Mumber 144110-42-9 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,4,1,1 and 0 respectively; the second part has 2 digits, 4 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 144110-42:
(8*1)+(7*4)+(6*4)+(5*1)+(4*1)+(3*0)+(2*4)+(1*2)=79
79 % 10 = 9
So 144110-42-9 is a valid CAS Registry Number.
144110-42-9Relevant articles and documents
5-bromo-6-chloro-3-hydroxyindole-2-methyl formate compound and preparation method and application thereof
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Paragraph 0102-0104, (2020/07/21)
The invention relates to the field of organic synthesis, and discloses a 5-bromo-6-chloro-3-hydroxyindole-2-methyl formate compound and a preparation method and application thereof. The indole compound has a structure as shown in a formula (I), and the preparation method comprises the following steps: obtaining a compound as shown in a formula (III) from a compound as shown in a formula (II), andthen obtaining a compound as shown in a formula (I). The novel method for synthesizing the indole phenol glycosidase chromogenic substrate and the salt thereof by using the indole compound shown in the formula (I) has the advantages of wide substrate application range and higher yield.
