136132-67-7

Basic information

• Product Name: N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE
• Synonyms: N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE;Z-ARG-ARG-7-AMIDO-4-METHYL COUMARIN, DIHCL;Z-ARG-ARG-AMC 2HCL;Z-ARG-ARG-AMC HYDROCHLORIDE SALT;Z-ARG-ARG-MCA HYDROCHLORIDE;Z-ARG-ARG-MCA(HYDROCHLORIDE FORM);Z-L-ARGINYL-L-ARGININE-7-AMIDO-4-METHYLCOUMARIN 2HCL;Z-RR-AMC, 2HCL
• CAS NO: 136132-67-7
• Molecular Formula: C30H40ClN9O6
• Molecular Weight: 658.15
• Product Categories: Substrates
• Mol File: 136132-67-7.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: −20°C
• CAS DataBase Reference: N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE(136132-67-7)
• EPA Substance Registry System: N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE(136132-67-7)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• Hazardous Substances Data: 136132-67-7(Hazardous Substances Data)

Usage

Description

N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is a synthetic compound designed as a substrate for specific protease enzymes, particularly cathepsin B and matrix metalloproteinase. It is characterized by its ability to emit fluorescence upon cleavage by these enzymes, making it a valuable tool in various research and diagnostic applications.

Uses

Used in Research and Diagnostic Applications:
N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used as a fluorogenic peptide substrate for the determination of cathepsin B and matrix metalloproteinase enzyme activities. Its fluorescence emission upon cleavage allows for the accurate measurement and analysis of these protease activities in various samples.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used as a tool for the development and testing of new drugs targeting cathepsin B and matrix metalloproteinase enzymes. These enzymes are involved in various pathological processes, including cancer and inflammatory diseases, making them important therapeutic targets.
Used in Biotechnology:
N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is also utilized in biotechnology for the study of protein-protein interactions and the development of biosensors for detecting specific protease activities. Its fluorescence properties make it an ideal candidate for these applications.
Used in Medical Diagnostics:
In medical diagnostics, N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE can be employed as a component in diagnostic assays to detect and monitor the activity of cathepsin B and matrix metalloproteinase enzymes in patient samples. This can help in the diagnosis and prognosis of various diseases associated with the dysregulation of these proteases.
Used in Enzyme Assay Development:
N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used as a substrate in the development of enzyme assays for cathepsin B and matrix metalloproteinase. These assays are essential for understanding the role of these enzymes in biological processes and for the development of inhibitors or activators that can modulate their activity.

Biochem/physiol Actions

Cathepsin B Active human primarily is a preproenzyme, glycosylated on rough endoplasmic reticulum. Processing of procathepsin B to a 31 kDa, single chain active form occurs in the lysosome. It is further processed to double chain form with a 25/26 kDa heavy chain and 5 kDa heavy chain in lysosome. Cathepsin B, functions as an exopeptidase at low pH. At higher pH in endosomes or neutral pH, cathepsin B functions as endopeptidase. Cathepsin B expression is upregulated in malignant tumors and facilitates tumor invasion. Cathepsin B might reduce the production of amyloid-β peptide associated with Alzheimerβs disease. It also plays a key role in neuroprotective functions.