129830-38-2 Usage
Description
Y-27632 (hydrochloride), also known as Y-27632 dihydrochloride, is a specific inhibitor of the Rho-associated protein kinase (ROCK) family. It is a cell-permeable compound that enhances stem cell survival and proliferation in culture. Y-27632 (hydrochloride) has been found to be an effective bronchodilator, improving lung resistance induced by antigen and acetylcholine, and controlling neutrophil migration and lung edema, making it a potential drug for treating acute lung injury.
Uses
1. Used in Pharmaceutical Applications:
Y-27632 (hydrochloride) is used as an inhibitor of ROCK-1 and ROCK-2 for enhancing stem cell survival and proliferation in culture. It significantly improves the freeze/thaw survival rate for human embryonic stem cells without influencing morphology, karyotype, cell surface markers, or differentiation potential.
2. Used in Organoid Culture:
Y-27632 (hydrochloride) is used as a medium supplement in pancreatic ductal adenocarcinoma organoid culture, aiding in the growth and maintenance of these organoids for research purposes.
3. Used in Stem Cell Research:
Y-27632 (hydrochloride) is used as an inhibitor of Rho kinase in mouse embryonic stem cells, human embryonic stem cells, and human induced pluripotent stem cells (iPSCs), facilitating the study of stem cell biology and potential therapeutic applications.
4. Used in Respiratory Medicine:
Y-27632 (hydrochloride) is used as a bronchodilator for improving lung resistance induced by antigen and acetylcholine, controlling neutrophil migration and lung edema, and potentially treating acute lung injury.
5. Used in the Inhibition of Rho-associated Protein Kinase (ROCK):
Y-27632 (hydrochloride) is used as an inhibitor of ROCK in various cell types, including mouse embryonic stem cells, human embryonic stem cells, and human induced pluripotent stem cells (iPSCs), to study the role of ROCK in cellular processes and its potential as a therapeutic target.
Biochem/physiol Actions
Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. Ki = 140 nM for p160ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.
References
1) Ishizaki et al. (2000), Pharmacological Properties of Y-27632, a Specific Inhibitor of Rho-Associated Kinases; Mol. Pharmacol., 57 976
2) Gauthaman et al. (2010), Effects of ROCK Inhibitor Y-27632 on Normal and Variant Human Embryonic Stem Cells (hESCs) In Vitro: Its Benefits in hESC Expansion; Stem Cell Rev., 6 86
3) Li et al. (2008), The ROCK Inhibitor Y-27632 enhances the Survival Rate of Human Embryonic Stem Cells Following Cryopreservation; Stem Cells Dev., 17 1079
4) Morel et al. (2018), Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature Y-27632; Mol. Cell. Proteomics, 17 1750 [Focus Citation]
5) Yamazaki et al. (2020) Chromatin condensation retains the osteogenic transcription factor, RUNX2, in the nucleus of human mesenchymal stem cells; J. Biomechanical Science and Engineering 15? 1 [Focus Citation]
Check Digit Verification of cas no
The CAS Registry Mumber 129830-38-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,9,8,3 and 0 respectively; the second part has 2 digits, 3 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 129830-38:
(8*1)+(7*2)+(6*9)+(5*8)+(4*3)+(3*0)+(2*3)+(1*8)=142
142 % 10 = 2
So 129830-38-2 is a valid CAS Registry Number.
129830-38-2Relevant articles and documents
A practical synthesis of Rho-Kinase inhibitor Y-27632 and fluoro derivatives and their evaluation in human pluripotent stem cells
Paleek, Jii,Zweigerdt, Robert,Olmer, Ruth,Martin, Ulrich,Kirschning, Andreas,Draeger, Gerald
, p. 5503 - 5510 (2011/08/10)
A practical synthesis of the Rho-Kinase inhibitor Y-27632 and two new fluoro derivatives was achieved in seven steps and with a good overall yield of 45% starting from commercially available (R)-1-phenylethylamine. Compared to Y-27632 the new fluoro deriv
Synthesis and evaluation of 4-(1-aminoalkyl)-N-(4-pyridyl) cyclohexanecarboxamides as Rho kinase inhibitors and neurite outgrowth promoters
Gingras, Karine,Avedissian, Hovsep,Thouin, Eryk,Boulanger, Véronique,Essagian, Charles,McKerracher, Lisa,Lubell, William D.
, p. 4931 - 4934 (2007/10/03)
The influence of stereochemistry and alkyl side chain length on the bioactivity of the Rho kinase inhibitor Y-27632 [(+)-1, R = Me] was examined by the synthesis of (+)- and (-)-1, and two alkyl chain analogs (+)- and (-)-2 (R = n-propyl) and (-)-3 (R = n
