1073244-85-5

Basic information

• Product Name: C₁₂H₁₁⁽²⁾H₈NO₂
• CAS NO: 1073244-85-5
• Molecular Weight: 217.225
• Mol File: 1073244-85-5.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: C₁₂H₁₁⁽²⁾H₈NO₂(CAS DataBase Reference)
• NIST Chemistry Reference: C₁₂H₁₁⁽²⁾H₈NO₂(1073244-85-5)
• EPA Substance Registry System: C₁₂H₁₁⁽²⁾H₈NO₂(1073244-85-5)

Safety Data

• Hazardous Substances Data: 1073244-85-5(Hazardous Substances Data)

Upstream product

• 51-61-6 dopamine 
• 1632-89-9 acetaldehyde-d4 

Relevant articles and documents

Diethylation labeling combined with UPLC/MS/MS for simultaneous determination of a panel of monoamine neurotransmitters in rat prefrontal cortex microdialysates

The primary challenge associated with the development of an LC/MS/MS-based assay for simultaneous determination of biogenic monoamine neurotransmitters such as norepinephrine (NE), dopamine (DA), serotonin (5-HT), and normetanephrine (NM) in rat brain microdialysates is to improve detection sensitivity. In this work, a UPLC/MS/MS-based method combined with a diethyl labeling technique was developed for simultaneous determination of a panel of monoamines in rat prefrontal cortex microdialysates. The chromatographic run time is 3.5 min/sample. The limits of detection of the UPLC/MS/MS-based method for NE, DA, 5-HT/ and NM, with/without diethyl labeling of monoamines, are 0.005/0.4 (30/2367 pM), 0.005/0.1 (33/653 pM), 0.005/0.2 (28/1136 pM), and 0.002/0.2 ng/mL (11/1092 pM), respectively. Diethyl labeling of amino groups of monoamines affords 20-100 times increased detection sensitivity of corresponding native monoamines during the UPLC/MS/MS analysis. This could result from the following: (1) improved fragmentation patterns; (2) increased hydrophobicity and concomitantly increased ionization efficiency in ESI MS and MS/MS analysis; (3) reduced matrix interference. This labeling reaction employs a commercially available reagent, acetaldehyde-d4, to label the amine groups on the monoamines via reductive animation. It is also simple, fast (～25-min reaction time), specific, and quantitative under mild reaction conditions. Data are also presented from the application of this assay to monitor the drug-induced changes of monoamine concentrations in rat prefrontal cortex microdialysate samples followed by administration of SKF 81297, a selective D1 dopamine receptor agonist known to elevate the extracellular level of the neurotransmitters DA and NE in the central nervous system.