10127-02-3 Usage
Description
Basic Orange 14, also known as Acridine Orange, is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It is characterized by its brown powder form and allows for visual detection of nucleic acids on agarose and polyacrylamide gels.
Uses
Used in Biomedical Research:
Basic Orange 14 is used as a fluorescent stain for nucleic acids, playing an important role in cell cycle determination and analysis of mitochondria and lysosomal content by flow cytometry.
Used as an RNA Polymerase Inhibitor:
Basic Orange 14 is used as an inhibitor of RNA polymerase, which can be useful in studying the regulation of gene expression and the mechanisms of RNA synthesis.
Used in Cell-Cycle Studies:
Basic Orange 14 is used as a nucleic acid-selective dye for cell-cycle studies, helping researchers understand the progression of cells through different phases of the cell cycle and identify potential targets for cancer therapy.
Used in Fluorescence and Epifluorescence Microscopy:
Basic Orange 14 is used as a cell-permeable dye in fluorescence and epiflouresence microscopy, enabling the visualization of nucleic acids within cells and aiding in the study of various cellular processes.
Used in Analyzing Multidrug Resistance:
Basic Orange 14 is used to characterize multidrug resistance, which can help in the development of strategies to overcome resistance to chemotherapy in cancer treatment.
Used in Measuring Changes in Mitochondrial Mass:
Basic Orange 14 is used to measure changes in mitochondrial mass during apoptosis in rat thymocytes, providing insights into the role of mitochondria in cell death and potential therapeutic targets for various diseases.
Biochem/physiol Actions
Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test.
Check Digit Verification of cas no
The CAS Registry Mumber 10127-02-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,1,2 and 7 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 10127-02:
(7*1)+(6*0)+(5*1)+(4*2)+(3*7)+(2*0)+(1*2)=43
43 % 10 = 3
So 10127-02-3 is a valid CAS Registry Number.
InChI:InChI=1/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
