2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate

Base Information

• Chemical Name: 2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate
• CAS No.: 10191-18-1
• Molecular Formula: C6H15NO5S
• Molecular Weight: 213.255
• Hs Code.: 29221980
• Mol file: 10191-18-1.mol

Synonyms:CHEBI:39045;2-[bis(2-hydroxyethyl)ammonio]ethanesulfonate

Chemical Property of 2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate

Chemical Property:

• Appearance/Colour: White/clear powder
• Vapor Pressure: 0.001Pa at 25℃
• Melting Point: 150-155 °C
• Refractive Index: 1.548
• PKA: 7.1(at 25℃) 7.15(at 20℃)
• PSA: 106.45000
• Density: 1.437 g/cm³
• LogP: -0.75840
• Storage Temp.: Store at RT.
• Solubility.: H2O: 1 M at 20 °C, clear, colorless
• Water Solubility.: Soluble
• XLogP3: -4.6
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 6
• Exact Mass: 213.06709375
• Heavy Atom Count: 13
• Complexity: 190

Purity/Quality:

99% ^*data from raw suppliers

BES ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi
• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: C(CO)[NH+](CCO)CCS(=O)(=O)[O-]
• Description: BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.The effect of BES and other zwitterionic buffers on the bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.
• Uses: BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C. Absorbance (100 mM, 260 nm):≤0.05.BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations. BES is used as a buffer to maintain the pH of the solutions in biological experiments. It is useful for diagnostic assay manufacturing industry. It induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. It acts as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers. BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.

Technology Process of 2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate

There total 6 articles about 2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 90.0%

2-[bis(2-hydroxyethyl)-amino]-ethanesulfonic acid 4-(tert-butyldiphenylsilanyloxy)-2,2-dimethylbutyl ester → 2-(di(2-hydroxyethyl)amino)ethanesulfonic acid (10191-18-1)

Guidance literature:

With tetrabutyl ammonium fluoride; In tetrahydrofuran; at 20 ℃; for 48h;

DOI:10.1039/b614333d

Reference yield: 80.0%

sodium N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonate (66992-27-6) → 2-(di(2-hydroxyethyl)amino)ethanesulfonic acid (10191-18-1)

Guidance literature:

With water;

Reference yield:

sodium bromoethanesulphonate + ethanolamine (141-43-5) → 2-(di(2-hydroxyethyl)amino)ethanesulfonic acid (10191-18-1)

Guidance literature:

With hydrogenchloride; In water;

upstream raw materials:

oxirane

Tau

ethene-sulfonate d'isopropyle

2,2'-iminobis[ethanol]

Downstream raw materials:

[Cu₄(N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid(-2H))4(4-HOC₆H₄COO)(Na(H₂O)4)](n)

[Cu₄(N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid(-2H))4(2-MeOC₆H₄COO)(Na(H₂O)2)](n)*nH₂O

N,N-bis(2-chloroethyl)aminoethanesulfonic acid

Refernces

• 1、 -. "A biological buffer preparation method". Paragraph 0069; 0070. . Retrieved 2017/08/02.
• 2、 -. "Isolation of nucleic acids". . . Retrieved 2008/06/13.