Fluorescein isothiocyanate

Base Information

• Chemical Name: Fluorescein isothiocyanate
• CAS No.: 27072-45-3
• Molecular Formula: C₂₁H₁₁NO₅S
• Molecular Weight: 389.388
• Hs Code.: 29321900
• Mol file: 27072-45-3.mol

Synonyms:Fluorescein,isothiocyanato- (6CI,8CI); FITC; Fluorescein isothiocyanate

Chemical Property of Fluorescein isothiocyanate

Chemical Property:

• Vapor Pressure: 9.53E-21mmHg at 25°C
• Melting Point: >360 °C(lit.)
• Boiling Point: 708.6°Cat760mmHg
• Flash Point: 382.4°C
• PSA: 120.44000
• Density: 1.54g/cm³
• LogP: 4.40010
• Storage Temp.: 2-8°C
• Solubility.: DMSO (Slightly), Methanol (Slightly)

Purity/Quality:

99.0% ^*data from raw suppliers

Fluorescein Isothiocyanate ^*data from reagent suppliers

Safty Information:

• Hazard Codes: Xn
• Statements: 42/43-36/37/38-20/21/22-42
• Safety Statements: 22-36-26-24/25-45-36/37

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Description: Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)Excitation source: 488 nm spectral line, argon-ion laserExcitation wavelength: 494 nmEmission wavelength: 518 nmExtinction coefficient: > 70,000 M-1cm-1Solubility: Soluble in aqueous buffers at pH > 6Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0
• Uses: Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites. For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.Label antibodies for use as immunofluorescent probes.Label oligonucleotides for hybridization probes.Detect proteins in gels and on Western blots.

Technology Process of Fluorescein isothiocyanate

There total 11 articles about Fluorescein isothiocyanate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 83.59%

carbon disulfide (75-15-0,12122-00-8) + fluoresceinamine (3326-34-9) → fluorescein isothiocyanate (3326-32-7,27072-45-3)

Guidance literature:

carbon disulfide; fluoresceinamine; With triethylamine; In methanol; at 5 - 20 ℃; for 8h;

With copper(l) chloride; In methanol; for 2h; Temperature;

Reference yield:

C21H13NO5S2*C6H15N → fluorescein isothiocyanate (3326-32-7,27072-45-3)

Guidance literature:

With benzenesulfonyl chloride; In tetrahydrofuran; at 30 ℃; Large scale;

Reference yield:

fluorescein (2321-07-5,126605-73-0) → fluorescein isothiocyanate (3326-32-7,27072-45-3)

Guidance literature:

With ammonia; In water;

upstream raw materials:

fluorescein

3',6'-dihydroxy-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

4-nitrophthalic acid

recorcinol

Downstream raw materials:

1-(2-aminoethyl)-3-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-yl)thiourea

8-{carboxy-[2-(fluoresceinylthioureido)ethyl]amido}octyl α-D-mannopyranoside

Refernces

• 1、 -. "REAGENT KIT USED FOR DETECTING GASTRIN-17, AND PREPARATION METHOD AND APPLICATION FOR REAGENT KIT". . . Retrieved 2018/09/02.
• 2、 -. "Single label comparative hybridization". . . Retrieved 2015/01/06.